Isolation and structural characterization of sulfated polysaccharide from Spirulina platensis and its bioactive potential: In vitro antioxidant, antibacterial activity and Zebrafish growth and reproductive performance

被引:72
|
作者
Rajasekar, Periyannan [1 ]
Palanisamy, Subramanian [2 ]
Anjali, Ravichandran [1 ]
Vinosha, Manoharan [1 ]
Elakkiya, Meyyanathan [1 ]
Marudhupandi, Thangapandi [3 ]
Tabarsa, Mehdi [4 ]
You, SangGuan [2 ]
Prabhu, Narayanasamy Marimuthu [1 ]
机构
[1] Alagappa Univ, Dept Anim Hlth & Management, Dis Control & Prevent Lab, Karaikkudi 630003, Tamil Nadu, India
[2] Gangneung Wonju Natl Univ, Dept Marine Food Sci & Technol, 120 Gangneungdaehangno, Kangnung 210702, Gangwon, South Korea
[3] Alagappa Univ, Biomat & Biotechnol Anim Hlth Lab, Dept Anim Hlth & Management, Sci Campus, Karaikkudi 630003, Tamil Nadu, India
[4] Tarbiat Modares Univ, Dept Seafood Proc, Fac Marine Sci, POB 46414-356, Noor, Iran
基金
新加坡国家研究基金会;
关键词
Spirulina platensis; Sulfated polysaccharide; Pharmaceutical activities; RADICAL-SCAVENGING ACTIVITY; NATURAL ANTIOXIDANTS; BROWN; PURIFICATION; EXTRACTION; FUCOIDAN; VIRUS; INGREDIENTS; INFECTIONS; IMPAIRMENT;
D O I
10.1016/j.ijbiomac.2019.09.024
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this study, the sulfated polysaccharide (SPs) was isolated from Spirulina platensis. The isolated SPs contains carbohydrate, sulfate, protein and uronic acid at 38.7 +/- 0.30%, 21.3 +/- 0.87%, 7.1 +/- 0.15% and 7.9 +/- 0.4% respectively. The elemental analysis confirmed the presence of carbon (18.01 +/- 0.10%), hydrogen (1.83 +/- 0.02%) and nitrogen (3.43 +/- 0.01%). The monosaccharide composition and molecular weight of SPs were analyzed by high-performance liquid chromatography and size exclusion chromatography respectively. The monosaccharide composition analysis showed the existence of glucose, rhamnose, xylose, fucose, mannose, galactose and the molecular weight of SPs was 1016 kDa. Further, the characterization of SPs was done by UV-visible spectroscopy, X-ray diffraction, FT-IR, H-1 NMR and C-13 NMR analysis. The obtained SPs exhibited potent antioxidant activity in DPPH (76.45 +/- 0.49%), reducing power (absorbance: 1.3 +/- 0.02), hydrogen peroxide scavenging (6.63 +/- 1.16%), hydroxyl scavenging (68.6 +/- 3.2%), nitric oxide (81.36 +/- 1.85%) and total antioxidant (absorbance:1.66 +/- 0.02) activities at 5 mg/ml. In addition, SPs revealed the highest antibacterial efficacy against the pathogenic bacteria Vibrio vulnificus in disc diffusion, agar bioassay and protein leakage assays at 100 mu g/ml. Furthermore, the supplementation of 2% SPs through a feed to the Danio rerio fish enhances the growth and reproductive performances. This finding confirmed that the isolated SPs from S. platensis possess pharmaceutical as well as nutritional properties. (C) 2019 Elsevier B.V. All rights reserved.
引用
收藏
页码:809 / 821
页数:13
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