LONG-TERM IN VITRO STORAGE AND MULTIPLICATION OF LEUCOJUM AESTIVUM L.

被引：2
|
作者：
Georgieva, L. [1 ]
Atanassov, A. [2 ]
Davidkova, L. [1 ]
Kondakova, V. [1 ]
机构：
[1] AgroBioInst, Sofia, Bulgaria
[2] Joint Genome Ctr, Sofia, Bulgaria
来源：
BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT | 2010年 / 24卷 / 03期
关键词：
Leucojum aestivum; in vitro; gene bank; galanthamine; GALANTHAMINE PRODUCTION; SUSPENSION-CULTURES; ALKALOIDS; SUCROSE; GROWTH; AJMALICINE;
D O I：
10.2478/V10133-010-0067-6
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Leucojum aestivum L. is a threatened medicinal plant used as a source of galanthamine in Bulgaria. The AChE inhibitor galanthamine is applied for treatment of Alzeimer's disease and other neurological disorders. The in vitro storage of genotype accessions from different natural populations with proven alkaloid profiles is of fundamental importance for the protection and rapid propagation of this threatened medicinal plant for both industry and natural resource protection. In this paper we describe a three step protocol for in vitro long-term conservation of L. aestivum- induction, regeneration and storage of explants. A gen-bank in AgroBioInstitute with accessions from 31 Bulgarian populations was created by us with the use of this in vitro protocol.
引用
收藏
页码：1950 / 1954
页数：5
相关论文
共 50 条
  • [41] Long-term storage of Clonorchis sinensis metacercariae in vitro
    Shunyu Li
    Hyoun Woo Kang
    Min-Ho Choi
    Sung-Tae Hong
    Parasitology Research, 2006, 100 : 25 - 29
  • [42] MONTHLY VARIATION OF PHARMACEUTICALLY VALUABLE ALKALOIDS, GALANTHAMINE AND LYCORINE, IN SUMMER SNOWFLAKE (LEUCOJUM AESTIVUM L.)
    Arslan, Mustafa
    Yildirim, Arzu Birinci
    Ozkan, Erva
    Oktelik, Fatma Betul
    Turker, Arzu Ucar
    FRESENIUS ENVIRONMENTAL BULLETIN, 2020, 29 (4A): : 2670 - 2677
  • [43] Effects of Long-Term Storage on Highbush Blueberry (Vaccinium corymbosum L.) Antioxidant Quality
    Cocetta, G.
    Mignani, I.
    Spinardi, A.
    XI INTERNATIONAL CONTROLLED AND MODIFIED ATMOSPHERE RESEARCH CONFERENCE, 2015, 1071 : 477 - 482
  • [44] Carbohydrate Changes in Parsnip (Pastinaca sativa L.) during Long-Term Cold Storage
    Ilic, Z. S.
    Sunic, L.
    V INTERNATIONAL CONFERENCE POSTHARVEST UNLIMITED, 2015, 1079 : 667 - 674
  • [45] Evaluating of hermetic bags for long-term storage of turmeric (Curcuma longa L.) rhizomes
    Yewle, Nileshwari R.
    Swain, Kishore C.
    Mann, Sandeep
    Dhakre, Digvijay Singh
    JOURNAL OF STORED PRODUCTS RESEARCH, 2021, 92
  • [46] Long-term storage of service tree (Sorbus domestica L.) seeds and induction of their germination
    Czech University of Life Sciences Prague, Faculty of Forestry and Wood Sciences, Kamýcká 1176, Prague 6-Suchdol
    165 21, Czech Republic
    J. For. Sci., 10 (417-421):
  • [47] Cryopreservation of cocoa (Theobroma cacao L.) somatic embryos for long-term germplasm storage
    Fang, JY
    Wetten, A
    Hadley, P
    PLANT SCIENCE, 2004, 166 (03) : 669 - 675
  • [48] Effect of long-term storage on viability of buffel grass (Cenchrus ciliaris L.) seeds
    da Silva, Jamille Cardeal
    Silva, Jailton de Jesus
    da Silva, Simonica Sousa
    Gomes, Raquel Araujo
    Dantas, Barbara Franca
    JOURNAL OF SEED SCIENCE, 2022, 44
  • [49] Effects of Long-Term Frozen Storage on Electrophoretic Patterns, Immunoreactivity, and Pepsin in Vitro Digestibility of Soybean (Glycine max L.) Proteins
    Sathe, Shridhar K.
    Sharma, Girdhari M.
    Kshirsagar, Harshal H.
    Su, Mengna
    Roux, Kenneth H.
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2009, 57 (04) : 1312 - 1318
  • [50] Carbohydrates stimulated Amaryllidaceae alkaloids biosynthesis in Leucojum aestivum L. plants cultured in RITA® bioreactor
    Ptak, Agata
    Moranska, Emilia
    Skrzypek, Edyta
    Warchol, Marzena
    Spina, Rosella
    Laurain-Mattar, Dominique
    Simlat, Magdalena
    PEERJ, 2020, 8
12345