Enantioseparation of the compounds of technical toxaphene (CTTs) on 35% heptakis(6-O-tert-butyldimethylsilyl-2,3-di-O-methyl)-β-cyclodextrin in OV1701

The separation of the enantiomers of the compounds of technical toxaphene (CTTs) on heptakis(6-O-tert-butyldimethylsilyl-2,3-di-O-methyl)-beta-cyclodextrin (beta-TBDM) has been studied by gas chromatography with electron-capture detection (GC-ECD). Enantiomers of eight of the nine CTTs under investigation were separated on this chiral stationary phase. Separations of the enantiomers of CTTs have hitherto been achieved on tert-butyldimethylsilylated beta-cyclodextrin (beta-BSCD). The chiral resolution values and separation factors of the CTTs on beta-TBDM have been compared with those obtained on beta-BSCD. Although several components coeluted, enantioselective determination of three CTTs was possible in an extract of seal blubber. For each CTT the first-eluting enantiomer was enantioenriched. Enantioselective accumulation of 2-endo,3-exo,5-endo,6-exo,8,8,9,10- octachlorobornane (B8-1412) in biota has been established for the first time.