The application of response surfaces for optimization of SERRS detection in a micro-fluidic device

Surface enhanced Raman resonance spectrometry (SERRS) using the surfaces of flowing colloidal particles provides a highly sensitive detection method for microfluidic systems. The sensitivity and reproducibility of the systems critically depends on the flow rates of the dye, colloid and aggregating agent in the microfluidic system. Optimization of these parameters was achieved using experimental design response surfaces to minimize the number of experiments required by augmenting small and large designs into one response surface. Using experimental design a linear calibration graph was obtained for cresyl violet acetate down to the 2 x 10(-9)M level.