Effects of Decontamination, DNA Extraction, and Amplification Procedures on the Molecular Diagnosis of Mycobacterium ulcerans Disease (Buruli Ulcer)

被引：9

作者：

Affolabi, Dissou ^{[2
]}

Sanoussi, N'Dira ^{[2
]}

Vandelannoote, Koen ^{[1
]}

Odoun, Mathieu ^{[2
]}

Faihun, Frank ^{[2
]}

Sopoh, Ghislain ^{[3
]}

Anagonou, Severin ^{[2
]}

Portaels, Francoise ^{[1
]}

Eddyani, Miriam ^{[1
]}

机构：

[1] Inst Trop Med, B-2000 Antwerp, Belgium

[2] Lab Reference Mycobacteries, Cotonou, Benin

[3] Ctr Depistage & Traitement Ulcere Buruli, Allada, Benin

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2012年 / 50卷 / 04期

关键词：

LABORATORY DIAGNOSIS; PCR; SPECIMENS;

D O I：

10.1128/JCM.05592-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We compared two DNA extraction methods (a semiautomated method using a Maxwell kit and a modified Boom method) and three amplification procedures (a single-step PCR, a nested PCR, and a real-time quantitative PCR) on 74 surgical tissue specimens from patients with clinically suspected Buruli ulcer. All of these procedures were compared before and after decontamination. We observed that, among the procedures tested, real-time PCR after the modified Boom extraction method or a single-run PCR assay after the Maxwell 16 extraction method, performed on nondecontaminated suspensions, are the best for the molecular diagnosis of Mycobacterium ulcerans disease.

引用

页码：1195 / 1198

页数：4

共 50 条

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