Novel Nested Direct PCR Technique for Malaria Diagnosis Using Filter Paper Samples

被引：52

作者：

Fuehrer, Hans-Peter ^{[1
,2
]}

Fally, Markus A. ^{[1
,2
]}

Habler, Verena E. ^{[1
,2
]}

Starzengruber, Peter ^{[1
,2
]}

Swoboda, Paul ^{[1
,2
]}

Noedl, Harald ^{[1
,2
]}

机构：

[1] Med Univ Vienna, Dept Specif Prophylaxis & Trop Med, A-1090 Vienna, Austria

[2] Malaria Res Initiat Bandarban MARIB, Bandarban, Bangladesh

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 04期

关键词：

D O I：

10.1128/JCM.01792-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The use of direct nested PCR enables the detection of Plasmodium spp. from blood samples collected on filter papers without requiring the time-consuming procedures associated with DNA extraction. Direct PCR provides a rapid, highly sensitive, and cost-effective alternative to diagnosing malaria using filter paper samples and standard nested PCR.

引用

页码：1628 / 1630

页数：3

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