Isolation and characterization of microsatellite loci in the tropical tree Jacaranda copaia (Bignoniaceae)

被引：12
|
作者：
Jones, FA [1 ]
Hubbell, SP
机构：
[1] Univ Georgia, Dept Plant Biol, Athens, GA 30602 USA
[2] Smithsonian Trop Res Inst, Balboa, Panama
来源：
MOLECULAR ECOLOGY NOTES | 2003年 / 3卷 / 03期
关键词：
Jacaranda copaia; microsatellite; Panama; paternity and maternity analysis; tropical tree;
D O I：
10.1046/j.1471-8286.2003.00465.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We isolated 10 microsatellite loci for jacaranda copaia for direct measurement of seed and pollen movement within the 50 ha Forest Dynamics Plot on Barro Colorado Island, Panama, and for studies of population genetic structure and gene flow across the Isthmus of Panama. Enriched repeat libraries were screened for microsatellite repeats and polymorphic primer pairs were developed. A total of 110 adult individuals were screened for allelic diversity and loci showed high levels of heterozygosity. These loci show both high maternal and paternal exclusion probabilities.
引用
收藏
页码：403 / 405
页数：3
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