Specificity of randomly generated genomic DNA fragment probes on a DNA array

被引：0
|
作者：
Tobino, Tomohiro [1 ]
Kurisu, Futoshi [2 ]
Kasuga, Ikuro [1 ]
Furumai, Hiroaki [2 ]
机构：
[1] Univ Tokyo, Grad Sch Engn, Dept Urban Engn, Bunkyo Ku, Tokyo 1138656, Japan
[2] Univ Tokyo, Grad Sch Engn, Res Ctr Water Environm Technol, Bunkyo Ku, Tokyo 1138656, Japan
来源：
FEMS MICROBIOLOGY LETTERS | 2012年 / 328卷 / 01期
关键词：
random genomic fragment probes; DNA array; hybridization specificity; OLIGONUCLEOTIDE MICROARRAY; MICROBIAL COMMUNITY; HYBRIDIZATION; MICROORGANISMS; BACTERIA; CHIP;
D O I：
10.1111/j.1574-6968.2011.02486.x
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The use of randomly generated DNA fragment sequences as probes on DNA arrays offers a unique potential for exploring unsequenced microorganisms. In this study, the detection specificity was evaluated with respect to probe-target sequence similarity using genomic DNAs of four Pseudomonas strains. Genome fragments averaging 2000bp were found to be specific enough to discriminate 85-90% similarity under highly stringent hybridization conditions. Such stringent conditions compromised signal intensities; however, specific signals remained detectable at the highest stringency (at 75 degrees C hybridization) with negligible false negatives. These results suggest that, without any probe design or selection, genomic fragments can provide a reasonable specificity for microbial diagnostics or species delineation by DNADNA similarities.
引用
收藏
页码：86 / 89
页数：4
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