In vitro fertility and motility characteristics of frozen-thawed boar epididymal spermatozoa separated by Percoll

被引：18
|
作者：
Suzuki, K [1 ]
Nagai, T
机构：
[1] Hokkaido Univ, FSC, Sapporo, Hokkaido 0600811, Japan
[2] Tohoku Natl Agr Exptl Stn, Morioka, Iwate 0200123, Japan
来源：
THERIOGENOLOGY | 2003年 / 60卷 / 08期
关键词：
boar epididymal spermatozoa; IVF; motility analysis; Percoll separation;
D O I：
10.1016/S0093-691X(03)00115-8
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Frozen-thawed epididymal spermatozoa from four boars were separated through a Percoll gradient, and motility characteristics and in vitro fertility were assessed. Percoll-separated spermatozoa had a significantly higher percentage of motile and progressively motile spermatozoa than those that were not separated (P < 0.0001). However, there were no clear differences in other motility parameters between Percoll-separated and un-separated spermatozoa. Furthermore, sperm agglutination was decreased by Percoll separation (P < 0.05). The effects of Percoll separation on in vitro fertility of spermatozoa differed among boars. In addition, there were large differences in fertility between sperm samples in vitro. Sperm samples, which indicate highly motile and progressively motile, did not always show high in vitro fertility. Furthermore, there was no distinct pattern between fertility in vitro and motility parameters. There was no difference in fertility in vitro between Percoll-separated and un-separated spermatozoa from two of the four boars. However, in vitro fertility of Percoll-separated spermatozoa was higher than that of un-separated spermatozoa from the other two boars. (C) 2003 Elsevier Science Inc. All rights reserved.
引用
收藏
页码：1481 / 1494
页数：14
相关论文
共 50 条
  • [21] Effects of (-)-Epigallocatechin Gallate on the Motility and Penetrability of Frozen-Thawed Boar Spermatozoa Incubated in the Fertilization Medium
    Kaedei, Y.
    Naito, M.
    Naoi, H.
    Sato, Y.
    Taniguchi, M.
    Tanihara, F.
    Kikuchi, K.
    Nagai, T.
    Otoi, T.
    REPRODUCTION IN DOMESTIC ANIMALS, 2012, 47 (06) : 880 - 886
  • [22] Comparative analysis of in vitro characteristics of fresh and frozen-thawed urethral and epididymal spermatozoa from cats (Felis domesticus)
    Prochowska, Sylwia
    Nizanski, Wojciech
    Partyka, Agnieszka
    THERIOGENOLOGY, 2016, 86 (08) : 2063 - 2072
  • [23] Proacrosin activation mechanisms in capacitated and frozen-thawed boar spermatozoa
    Cui, J. J.
    Tian, Y.
    Liu, Y.
    Huang, H. L.
    Jin, Y.
    GENETICS AND MOLECULAR RESEARCH, 2014, 13 (04) : 9915 - 9920
  • [24] Ameliorative effect of silymarin on the quality of frozen-thawed boar spermatozoa
    Yue, Shunli
    Wang, Shunwei
    Liu, Xue
    Bian, Xiaoqi
    Ding, Chang
    Wu, Tong
    Li, Dantong
    Zhou, Jiabo
    REPRODUCTION IN DOMESTIC ANIMALS, 2023, 58 (02) : 298 - 306
  • [25] The effect of antioxidants on motility, viability, acrosome integrity and DNA integrity of frozen-thawed epididymal cat spermatozoa
    Thuwanut, P.
    Chatdarong, K.
    Techakumphu, A.
    Axner, E.
    THERIOGENOLOGY, 2008, 70 (02) : 233 - 240
  • [26] In vitro fertilizing capacity of frozen-thawed boar semen
    Cordova, A
    Ducolomb, Y
    Jimenez, I
    Casas, E
    Bonilla, E
    Betancourt, M
    THERIOGENOLOGY, 1997, 47 (07) : 1309 - 1317
  • [27] Developmental competence of Dromedary camel oocytes fertilized in vitro by frozen-thawed ejaculated and epididymal spermatozoa
    Scholkamy, T. H.
    El-Badry, D. A.
    Mahmoud, K. Gh. M.
    IRANIAN JOURNAL OF VETERINARY RESEARCH, 2016, 17 (04) : 253 - 258
  • [28] Intracytoplasmic sperm injection of in vitro matured oocytes of domestic cats with frozen-thawed epididymal spermatozoa
    Bogliolo, L
    Leoni, G
    Ledda, S
    Naitana, S
    Zedda, M
    Carluccio, A
    Pau, S
    THERIOGENOLOGY, 2001, 56 (05) : 955 - 967
  • [29] Effect of acrosome reaction progress in frozen-thawed boar spermatozoa on the efficiency of in vitro oocyte fertilization
    Martecikova, S.
    Hulinska, P.
    Reckova, Z.
    Pavlik, A.
    Jeseta, M.
    Machatkova, M.
    VETERINARNI MEDICINA, 2010, 55 (09) : 429 - 437
  • [30] In vitro fertilization using frozen-thawed feline epididymal spermatozoa from corpus and cauda regions
    Kunkitti, Panisara
    Axner, Eva
    Bergqvist, Ann-Sofi
    Sjunnesson, Ylva
    THERIOGENOLOGY, 2016, 86 (06) : 1403 - 1408
12345