Whey protein supplementation increases methionine intake but not homocysteine plasma concentration in rats

被引:8
|
作者
Deminice, Rafael [1 ,2 ]
Comparotto, Hugo [1 ]
Jordao, Alceu Afonso [1 ]
机构
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Lab Nutr & Metab, BR-14049 Ribeirao Preto, SP, Brazil
[2] Univ Estadual Londrina, Fac Phys Educ & Sport, Dept Phys Educ, Londrina, Parana, Brazil
关键词
protein supplements; hyperhomocysteinemia; oxidative stress; liver; glutathione; VASCULAR ENDOTHELIAL DYSFUNCTION; CREATINE SUPPLEMENTATION; OXIDATIVE STRESS; HYPERHOMOCYSTEINEMIA; REQUIREMENTS; GLUTATHIONE; METABOLISM; DIET;
D O I
10.1139/apnm-2014-0175
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
The purpose of this study was to examine the effects of whey protein supplementation on homocysteine (Hcy) metabolism and liver oxidative stress in rats. Twenty-four rats were divided into 3 groups (n = 8) to receive one of the following diets for 4 weeks: control diet (C), whey protein-composed diet (WP), and whey protein-supplemented diet (WPS). The C and WP diets consisted of AIN-93 with 20% casein and 20% whey protein as protein source, respectively. WPS was AIN-93 (20% casein) supplemented by the addition of 20% (w/w) whey protein. Four weeks of ingesting a WPS diet resulted in a significantly higher (P < 0.05) total protein and methionine intakes. Although a significant increase (P < 0.05) in the hepatic S-adenosylmethionine and S-adenosylhomocysteine levels occurred in WPS group compared with C and WP, no significant change was observed in plasma Hcy concentration between groups. Furthermore, the levels of lipid hydroperoxides and advanced oxidation protein products, known liver oxidative stress markers, were increased in the WPS group compared with the C group. In addition, no change in glutathione liver concentration was observed in any of the groups studied. In conclusion, whey protein supplementation increases methionine intake substantially; however, it does not change plasma Hcy concentrations. On the other hand, increased hepatic oxidative stress markers were observed in whey protein supplemented rats were probably due to high protein intake.
引用
收藏
页码:46 / 50
页数:5
相关论文
共 50 条
  • [41] A Protein Extract from Chicken Reduces Plasma Homocysteine in Rats
    Lysne, Vegard
    Bjorndal, Bodil
    Vik, Rita
    Nordrehaug, Jan Erik
    Skorve, Jon
    Nygard, Ottar
    Berge, Rolf K.
    NUTRIENTS, 2015, 7 (06): : 4498 - 4511
  • [42] Reduction in plasma homocysteine levels and lipid peroxidation through intake of multivitamin-multimineral supplementation
    Chae, JS
    Park, SH
    Kim, HJ
    Jang, Y
    Lee, JH
    Randolph, RK
    Davies, AJ
    ATHEROSCLEROSIS SUPPLEMENTS, 2003, 4 (02) : 279 - 279
  • [43] Supplementation with a mixed fruit and vegetable concentrate increases plasma antioxidant vitamins and lowers plasma homocysteine in men.
    Samman, S
    Sivarajah, G
    Man, J
    Ahmad, Z
    Petocz, P
    Caterson, I
    FASEB JOURNAL, 2002, 16 (04): : A238 - A238
  • [44] Effects of sheep/goat whey protein dietary supplementation on the redox status of rats
    Kerasioti, Efthalia
    Stagos, Dimitrios
    Tsatsakis, Aristides M.
    Spandidos, Demetrios A.
    Taitzoglou, Ioannis
    Kouretas, Demetrios
    MOLECULAR MEDICINE REPORTS, 2018, 17 (04) : 5774 - 5781
  • [45] Oral supplementation with whey proteins increases plasma glutathione levels of HIV-infected patients
    Micke, P
    Beeh, KM
    Schlaak, JF
    Buhl, R
    EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, 2001, 31 (02) : 171 - 178
  • [46] Effects of chronic betaine ingestion on methionine-loading induced plasma homocysteine elevation in rats
    Yagisawa, Masako
    Shigematsu, Norihiro
    Nakata, Ricko
    JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY, 2006, 52 (03) : 194 - 199
  • [47] Acute stressor-selective effect on total plasma homocysteine concentration in rats
    de Oliveira, AC
    Suchecki, D
    Cohen, S
    D'Almeida, V
    PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR, 2004, 77 (02) : 269 - 273
  • [48] Betaine supplementation suppresses plasma homocysteine level elevation induced by folate deficiency in rats
    Yagisawa, Masako
    Doi, Yuko
    Uenohara, Terumi
    Toda, Maiko
    Shigematsu, Norihiro
    Nakata, Rieko
    NUTRITION RESEARCH, 2006, 26 (06) : 266 - 270
  • [49] APOMORPHINE INCREASES PLASMA OXYTOCIN CONCENTRATION IN MALE-RATS
    MELIS, MR
    ARGIOLAS, A
    GESSA, GL
    NEUROSCIENCE LETTERS, 1989, 98 (03) : 351 - 355
  • [50] Oral nutritional supplementation increases caloric and protein intake in peritoneal dialysis patients
    Boudville, N
    Rangan, A
    Moody, H
    AMERICAN JOURNAL OF KIDNEY DISEASES, 2003, 41 (03) : 658 - 663