Roseomonas rosulenta sp. nov., isolated from rice paddy soil

Three bacterial isolates, Gram-stain-negative, non-motile, coccobacilli-shaped bacteria, strains OP-27(T), OP-5 and OP-30, were isolated from rice paddy soil. Phylogenetic analyses based on 16S rRNA gene sequences revealed that three isolates belonged to the genus Roseomonas, showing the highest sequence similarities to Roseomonas sediminicola FW-3(T) (98.1%) and Roseomonas lacus TH-G33(T) (98.0%). The genome size of strain OP-27(T) was 5.2 Mb in a single contig with DNA G+C content of 71.2%. The genome included 5164 predicted protein-coding genes, as well as 48 tRNA, 4 rRNA and 4 mRNA genes. The average nucleotide identity value between strain OP-27(T) and type strains of related species of the genus Roseomonas were 81.1-83.1%, and the digital DNA-DNA hybridization values of strain OP-27(T) and the related strains were 24.6-26.8%, respectively. The DNA-DNA hybridization values between strains OP-27(T), OP-5 and OP-30 were 84-100% and its closest relative, Roseomonas sediminicola KACC 16616(T) was 21.1%. The major fatty acids were C-18:1 omega 7c, C-18:1 2-OH and C-16:0 and predominant quinone was Q-10. Based on its distinctive phenotypic, phylogenetic, and chemotaxonomic characteristics, the three strains are considered to represent novel species of the genus Roseomonas, for which the name Roseomonas rosulenta sp. nov. is proposed. The type strain is OP-27(T) (=KACC 21501(T)= NBRC 114497(T)).