Falsiroseomonas oryziterrae sp. nov., and Falsiroseomonas oryzae sp. nov., isolated from rice paddy soil

Three Gram-stain-negative, aerobic, non-motile and coccobacilli-shaped bacterial strains, designated as NPKOSM-4(T), NPKOSM-8 and MO-31(T), were isolated from rice paddy soil. They had 96.5-100 % 16S rRNA gene sequence similarity to each other, and strains NPKOSM-4(T) and NPKOSM-8 showed 100 % 16S rRNA gene sequence similarity, confirming that they were the same species. Comparative analysis of 16S rRNA genes with closely related type strains showed that three isolates were most closely related to Falsiroseomonas terricola EM0302(T) (96.1-97.8 %), Falsiroseomonas wooponensis WW53(T) (95.51-96.3 %) and Falsiroseomonas bella CQN31(T) (96.0-96.5 %), respectively. The genomes of strains NPKOSM-4(T) and MO-31(T) consisted of 4 632 875 and 6 455 771 bps, respectively, with 72.0 and 72.1 mol% G+C content. The average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA-DNA hybridization (dDDH) values between strains NPKOSM-4(T) and MO-31(T) and type strains of Falsiroseomonas species were lower than the cut-offs (>= 95 % for ANI, >= 95-96 % for AAI and >= 70 % for dDDH) required to define a bacterial species. The major fatty acids of strains NPKOSM-4(T), NPKOSM-8 and MO-31(T) were C-18 : 1 omega 7c and C-18 : 1 2-OH (<10 %) and the predominant quinone was Q-10. The polar lipids of strain NPKOSM-4(T) were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, one unidentified aminophospholipid and three unidentified aminolipids. The polar lipid profiles of strain MO-31(T) contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, one unidentified aminolipid and three unidentified lipids. Based on their distinctive phenotypic, phylogenetic, and chemotaxonomic characteristics, strains NPKOSM-4(T), NPKOSM-8 and MO-31(T) are considered to represent two novel species of the genus Falsiroseomonas, for which the names Falsiroseomonas oryziterrae sp. nov. [to accommodate strains NPKOSM-4(T) (= KACC 22135(T)=JCM 34745(T)), NPKOSM-8 (=KACC 22134=JCM 34746)] and Falsiroseomonas oryzae sp. nov. [to accommodate strain MO-31(T) (= KACC 22465(T)=JCM 35532(T))] are proposed.