Recent advances in micro-scale and nano-scale high-performance liquid-phase chromatography for proteome research

被引：38
|
作者：
Tao, Dingyin [1 ,2 ]
Zhang, Lihua [1 ]
Shan, Yichu [1 ]
Liang, Zhen [1 ]
Zhang, Yukui [1 ]
机构：
[1] Chinese Acad Sci, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
来源：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2011年 / 399卷 / 01期
关键词：
Proteome; Micro-HPLC; Nano-HPLC; Multi-dimensional separation; MS-MS; TANDEM MASS-SPECTROMETRY; MALDI-TOF-MS; SIZE-EXCLUSION CHROMATOGRAPHY; HUMAN LIVER-TISSUE; REVERSED-PHASE; MONOLITHIC COLUMNS; ELECTROSPRAY-IONIZATION; SHOTGUN PROTEOMICS; ESI-MS/MS; MULTIDIMENSIONAL SEPARATIONS;
D O I：
10.1007/s00216-010-3946-7
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
High-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS-MS) is regarded as one of the most powerful techniques for separation and identification of proteins. Recently, much effort has been made to improve the separation capacity, detection sensitivity, and analysis throughput of micro- and nano-HPLC, by increasing column length, reducing column internal diameter, and using integrated techniques. Development of HPLC columns has also been rapid, as a result of the use of submicrometer packing materials and monolithic columns. All these innovations result in clearly improved performance of micro- and nano-HPLC for proteome research.
引用
收藏
页码：229 / 241
页数：13
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