Thermal spring water drinking attenuates dextran-sulfate-sodium-induced colitis in mice

The present study investigates the effect of oral consumption of hydrogen sulfide-containing Harkany thermal spring water, as well as sodium hydrogen sulfide (NaHS) solution on experimental colitis. Colitis was induced by 2 % dextran sulfate sodium (DSS) in the drinking water of C57BL/6 mice for 7 days. Some animal groups drank Harkany thermal spring water or water supplemented with 21.68 mg/L NaHS. General signs of colitis, myeloperoxidase (MPO) enzyme activity of colon samples, histological features of colitis and function of the enteric nervous system were assessed. Oral administration of Harkany thermal spring water significantly attenuated general signs of colitis, MPO enzyme activity of colon samples and detrimental effect of colitis on the function of the enteric nervous system, but not histological signs of colitis. These findings could be reproduced using NaHS solution with additional significantly diminished histological damage. We conclude that oral treatment with Harkany thermal spring water relieves various aspects of DSS-evoked colitis in mice. This effect is most likely to be mediated by hydrogen sulfide content of the Harkany water. Our data might promote complementary utilization of sulfurous thermal spring water in the therapy of inflammatory bowel disease.