Autophagy is essential for preimplantation development of mouse embryos

被引:458
|
作者
Tsukamoto, Satoshi [1 ]
Kuma, Akiko [1 ,2 ]
Murakami, Mirei [1 ]
Kishi, Chieko [1 ]
Yamamoto, Akitsugu [3 ]
Mizushima, Noboru [1 ,2 ]
机构
[1] Tokyo Med & Dent Univ, Dept Physiol & Cell Biol, Bunkyo Ku, Tokyo 1138519, Japan
[2] Japan Sci & Technol Agcy, SORST, Kawaguchi, Saitama 3320012, Japan
[3] Nagahama Inst Biosci & Technol, Dept Biosci, Nagahama 5260829, Japan
关键词
D O I
10.1126/science.1154822
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
After fertilization, maternal proteins in oocytes are degraded and new proteins encoded by the zygotic genome are synthesized. We found that autophagy, a process for the degradation of cytoplasmic constituents in the lysosome, plays a critical role during this period. Autophagy was triggered by fertilization and up- regulated in early mouse embryos. Autophagy- defective oocytes derived from oocyte- specific Atg5 ( autophagy- related 5) knockout mice failed to develop beyond the four- and eight- cell stages if they were fertilized by Atg5- null sperm, but could develop if they were fertilized by wild- type sperm. Protein synthesis rates were reduced in the autophagy- null embryos. Thus, autophagic degradation within early embryos is essential for preimplantation development in mammals.
引用
收藏
页码:117 / 120
页数:4
相关论文
共 50 条
  • [42] POSITIVE EFFECT OF TAURINE ON PREIMPLANTATION DEVELOPMENT OF MOUSE EMBRYOS INVITRO
    DUMOULIN, JCM
    EVERS, JLH
    BRAS, M
    PIETERS, MHEC
    GERAEDTS, JPM
    JOURNAL OF REPRODUCTION AND FERTILITY, 1992, 94 (02): : 373 - 380
  • [43] EFFECT OF DAMAGE TO THE ZONA PELLUCIDA ON DEVELOPMENT OF PREIMPLANTATION EMBRYOS IN THE MOUSE
    NICHOLS, J
    GARDNER, RL
    HUMAN REPRODUCTION, 1989, 4 (02) : 180 - 187
  • [44] EFFECT OF INHIBITORS OF PROTEIN SYNTHESIS ON DEVELOPMENT OF PREIMPLANTATION MOUSE EMBRYOS
    THOMSON, JL
    BIGGERS, JD
    EXPERIMENTAL CELL RESEARCH, 1966, 41 (02) : 411 - +
  • [45] The effects of glutamine and EDTA on the development of preimplantation mouse embryos in vitro
    Fung, CK
    Chan, STH
    Yeung, WSB
    HUMAN REPRODUCTION, 1999, 14 : 338 - 339
  • [46] Cleavage and development in cultured preimplantation mouse embryos exposed to lidocaine
    DelValle, LJ
    Orihuela, PA
    REPRODUCTIVE TOXICOLOGY, 1996, 10 (06) : 491 - 496
  • [47] BET proteins are essential for the specification and maintenance of the epiblast lineage in mouse preimplantation embryos
    Mami Tsume-Kajioka
    Chiharu Kimura-Yoshida
    Kyoko Mochida
    Yoko Ueda
    Isao Matsuo
    BMC Biology, 20
  • [48] BET proteins are essential for the specification and maintenance of the epiblast lineage in mouse preimplantation embryos
    Tsume-Kajioka, Mami
    Kimura-Yoshida, Chiharu
    Mochida, Kyoko
    Ueda, Yoko
    Matsuo, Isao
    BMC BIOLOGY, 2022, 20 (01)
  • [49] MOUSE INTERFERON PRODUCED INVIVO DOES NOT INHIBIT THE DEVELOPMENT OF PREIMPLANTATION MOUSE EMBRYOS
    CARTHEW, P
    WOOD, M
    KIRBY, C
    JOURNAL OF REPRODUCTION AND FERTILITY, 1986, 77 (01): : 75 - 79
  • [50] BIOPSY OF PREIMPLANTATION MOUSE EMBRYOS - DEVELOPMENT OF MICROMANIPULATED EMBRYOS AND PROLIFERATION OF SINGLE BLASTOMERES INVITRO
    WILTON, LJ
    TROUNSON, AO
    BIOLOGY OF REPRODUCTION, 1989, 40 (01) : 145 - 152