ATX-LPA Axis Induces Expression of OPN in Hepatic Cancer Cell SMMC7721

被引：12

作者：

Zhang, Rihua ^{[1
]}

Zhang, Zhihong ^{[2
]}

Pan, Xiaolin ^{[1
]}

Huang, Xiayue ^{[1
]}

Huang, Zuhu ^{[3
]}

Zhang, Guoxin ^{[1
]}

机构：

[1] Nanjing Med Univ, Affiliated Hosp 1, Dept Gastroenterol, Nanjing 210029, Peoples R China

[2] Nanjing Med Univ, Affiliated Hosp 1, Dept Pathol, Nanjing 210029, Peoples R China

[3] Nanjing Med Univ, Affiliated Hosp 1, Dept Infect Dis, Nanjing 210029, Peoples R China

来源：

ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY | 2011年 / 294卷 / 03期

基金：

中国国家自然科学基金;

关键词：

autotaxin; lysophosphatidylcholine; lysophosphatidic acid; osteopontin; ATX-LPA axis; LYSOPHOSPHATIDIC ACID; HEPATOCELLULAR-CARCINOMA; OVARIAN-CANCER; LYSOPHOSPHOLIPASE-D; BREAST-CANCER; OSTEOPONTIN; AUTOTAXIN; PROMOTES; INVASION; GROWTH;

D O I：

10.1002/ar.21324

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

Osteopontin (OPN) and autotaxin (ATX) are important chemokines involved in the survival, proliferation, migration, invasion, and metastasis of many cancer cells. The focus of the study was to investigate the relationship between OPN and ATX-lysophosphatidic acid (LPA) axis. The expression of OPN and its cellular cascades were determined by western blot and real-time quantitative transcription polymerase chain reaction (real-time PCR) analyses. Cell migration activity was determined by a Transwell-migration assay. In comparison with nontreated cells, we found that the ATX-LPA axis upregulated OPN expression by 2.91-fold in protein levels and 2.52-fold in mRNA levels. The ATX-LPA axis activates Akt and ATX/LPC-induced OPN expression in SMMC7721 cells was largely reduced by the inhibitors of phosphatidylinositol 3-kinase (PI3K)/Akt or LPA receptor. This study provides the first evidence that the induction of the OPN expression by ATX-LPA axis was mediated by the activation of Akt through LPA receptors and OPN was required for migration of SMMC7721 cells induced by ATX-LPA axis. Anat Rec, 294:406-411, 2011. (C) 2010 Wiley-Liss, Inc.

引用

页码：406 / 411

页数：6

共 34 条

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