Analysis of organochlorine pesticides in mollusc samples by HPLC after microwave assisted micellar extraction coupled with solid phase extraction

A mixture of six organochlorine pesticides were determined by HPLC in different types of molluscs after their extraction by microwave assisted micellar extraction (MAME) coupled with a clean-up and preconcentration step by solid phase extraction (SPE). A non-ionic surfactant (Polyoxyethylene 10 lauryl ether, POLE) was used in MAME followed by SPE for cleaning and preconcentration of the samples. The extracts were determined by HPLC-UV. With the optimum experimental variables established, the analytical parameters of the method were determined with satisfactory results (relative standard desviation values between 3.9 and 9.3% and detection limits between 6 and 38 ng . g(-1)). Themethodwas validated using the conventional Soxhlet extraction method.