Alcanivorax profundi sp. nov., isolated from deep seawater of the Mariana Trench

A Gram-stain-negative, rod-shaped, non-motile, strictly aerobic strain, designated as MTEO17(T), was isolated from a 1000 m deep seawater sample of the Mariana Trench. Growth was observed at 10-45 degrees C (optimum, 37 degrees C), in the presence of 0.0-12.0 % NaCl (w/v; optimum, 3.0 %) and at pH 6.0-10.0 (optimum, pH 7.0-8.0). Phylogenetic analysis, based on the 16S rRNA gene sequence, revealed that strain MTEO17(T)- belonged to the genus Alcanivorax and showed the highest sequence similarity of 97.9% to Alcanivorax nanhaiticus MCCC 1A05629(T). The estimated average nucleotide identity and DNA-DNA hybridization values between strain MTEO17(T)- and A. nanhaiticus MCCC 1A05629(T) were 78.98 and 23.80%, respectively. The significant dominant fatty acids were C-16:0, summed feature 8 (C-18:1 omega 6c and/or C-18:1 omega 7c) and summed feature 3 (C-16:1 omega 6c and/or C-16:1 omega 7c). The polar lipids comprised two phosphatidylethanolamines, one phosphatidylglycerol, one unidentified phospholipid and four unidentified polar lipids. The DNA G+C content of strain MTEO17(T)- was 57.5 %. On the basis of the polyphasic evidence, strain MTEO17(T)- is proposed to represent a novel species of the genus Alcanivorax, for which the name Alcanivorax profundi sp. nov. is proposed. The type strain is MTEO17(T)- (=KCTC 52694(T)=MCCC 1K03252(T)).