Sphingomonas profundi sp. nov., isolated from deep-sea sediment of the Mariana Trench

A Gram-stain-negative, short rod-shaped, yellow bacterium (strain LMO-1(T)) was isolated from deep-sea sediment of the Mariana Trench, Challenger Deep. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain LMO-1(T) belonged to genus Sphingomonas, with the highest sequence similarity to Sphingomonas formosensis CC-Nfb-2(T) (96.3 %), followed by Sphingomonas prati W18RD(T) (96.1%), Sphingomonas arantia 6P(T) (96.0 %) and Sphingomonas montana W16RD(T) (95.9%). The predominant polar lipids were phosphatidylethanolamine, sphingoglycolipid, phosphatidylglycerol and phosphatidylcholine. The main cellular fatty acids were summed feature 8 (C-18:1 omega 7c and/or C-18:1 omega 6c), C-16:0 and C-14:0 2-OH. The major polyamine was sym-homospermidine and the predominant isoprenoid quinone was ubiquinone-10. The genome DNA G+C content of strain LMO-1(T) was 69.2 mol%. The average nucleotide identity and DNA-DNA hybridization values between strain LMO-1(T) and CC-Nfb-2(T) were 75.9 and 20.5 %, respectively. Based on these data, LMO-1(T) should be classified as representing a novel species of the genus Sphingomonas, for which the name Sphingomonas profundi sp. nov. is proposed. The type strain is LMO-1(T) (=MCCC 1K04066(T)=JCM 33666(T)).