Identification of Yersinia enterocolitica using a random genomic DNA microarray chip

被引：5
|
作者：
Bang, J. [1 ]
Beuchat, L. R. [2 ,3 ]
Gu, M. B. [1 ]
Chang, H. -I. [1 ]
Ryu, J. -H. [1 ]
机构：
[1] Korea Univ, Grad Sch Life Sci & Biotechnol, Seoul, South Korea
[2] Univ Georgia, Ctr Food Safety, Griffin, GA USA
[3] Univ Georgia, Dept Food Sci & Technol, Griffin, GA 30223 USA
来源：
LETTERS IN APPLIED MICROBIOLOGY | 2010年 / 51卷 / 06期
关键词：
DNA chip; identification; randomly fragmented genomic DNA; Yersinia enterocolitica; PCR METHOD; SP-NOV; ENTEROBACTERIACEAE; HYBRIDIZATION; INFECTIONS; BACTERIA; SAMPLES; FOOD;
D O I：
10.1111/j.1472-765X.2010.02954.x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Aims: To fabricate a DNA chip containing random fragments of genomic DNA of Yersinia enterocolitica and to verify its diagnostic ability. Methods and Results: A DNA microarray chip was fabricated using randomly fragmented DNA of Y. enterocolitica. Chips were hybridized with genomic DNA extracted from other Y. enterocolitica strains, other Yersinia spp. and bacteria in different genera. Genomic DNA extracted from Y. enterocolitica showed a significantly higher hybridization rate compared with DNA of other Yersinia spp. or bacterial genera, thereby distinguishing it from other bacteria. Conclusions: A DNA chip containing randomly fragmented genomic DNA from Y. enterocolitica can detect Y. enterocolitica and clearly distinguish it from other Yersinia spp. and bacteria in different genera. Significance and Impact of the Study: A microarray chip containing randomly fragmented genomic DNA of Y. enterocolitica was fabricated without sequence information, and its diagnostic ability to identify Y. enterocolitica was verified.
引用
收藏
页码：665 / 670
页数：6
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