Effects of Granulocyte Colony-Stimulating Factor on Proliferation and Apoptosis of B Cells in Bone Marrow of Healthy Donors

被引:2
|
作者
Zhai, Shu-Zhen [1 ,2 ]
Guo, Hui-Dong [1 ,2 ]
Li, Si-Qi [1 ,2 ]
Zhao, Xiao-Su [1 ,2 ]
Wang, Yu [1 ,2 ]
Xu, Lan-Ping [1 ,2 ]
Liu, Kai-Yan [1 ,2 ]
Huang, Xiao-Jun [1 ,2 ]
Chang, Ying-Jun [1 ,2 ]
机构
[1] Peking Univ Peoples Hosp, Beijing, Peoples R China
[2] Peking Univ, Beijing Key Lab Hematopoiet Stem Cell Transplanta, Inst Hematol, Beijing, Peoples R China
关键词
VERSUS-HOST-DISEASE; G-CSF; HEMATOPOIETIC-CELLS; T-CELLS; MOBILIZATION; PUMA; GENE; LYMPHOPOIESIS; RECEPTOR;
D O I
10.1016/j.transproceed.2019.11.004
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Background and Objective. The aim of this study was to investigate the effects of granulocyte colony-stimulating factor (G-CSF) on the proliferation and apoptosis of bone marrow (BM) B cells from healthy donors and its mechanism. Materials and Methods. The proliferation ability and apoptosis of BM cells from healthy donors before and after in vivo G-CSF application were determined by multiparameter flow cytometry. The gene expression of B cells was detected by RNA-Seq. In vitro experiments were performed to investigate the effects of G-CSF on the proliferation and apoptosis of BM B cells through which gene. Results. Treating healthy donors with G-CSF significantly decreased proliferation and increased apoptosis of BM B cells. The proliferation of CD19(+)CD27(-) B cell subgroup and CD19(+)CD24(hi)CD38(hi) B cell subset were also decreased. G-CSF also significantly altered proapoptotic genes, cell cycle arrest genes, and DNA replication and cell cycle genes, especially significantly increased SOCS1 expression of BM B cells. In vitro experiments showed that SOCS1 overexpression did not affect B cell proliferation ability and apoptosis. Conclusions. Our results suggest that extensive effects of G-CSF on BM B cells, such as inhibiting proliferation, inducing apoptosis, and altering a series of gene expression.
引用
收藏
页码:345 / 352
页数:8
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