Anchor-based bisulfite sequencing determines genome-wide DNA methylation

被引：7

作者：

Chapin, Nathaniel ^{[1
]}

Fernandez, Joseph ^{[1
]}

Poole, Jason ^{[1
]}

Delatte, Benjamin ^{[1
]}

机构：

[1] Act Motif, Adv Res Lab, 1914 Palomar Oaks Way STE 150, Carlsbad, CA 92008 USA

来源：

COMMUNICATIONS BIOLOGY | 2022年 / 5卷 / 01期

关键词：

ACID;

D O I：

10.1038/s42003-022-03543-1

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Whole Genome Bisulfite Sequencing (WGBS) is the current standard for DNA methylation profiling. However, this approach is costly as it requires sequencing coverage over the entire genome. Here we introduce Anchor-Based Bisulfite Sequencing (ABBS). ABBS captures accurate DNA methylation information in Escherichia coli and mammals, while requiring up to 10 times fewer sequencing reads than WGBS. ABBS interrogates the entire genome and is not restricted to the CpG islands assayed by methods like Reduced Representation Bisulfite Sequencing (RRBS). The ABBS protocol is simple and can be performed in a single day.

引用

页数：6

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