Profiling the genome-wide DNA methylation pattern of porcine ovaries using reduced representation bisulfite sequencing

被引：34
|
作者：
Yuan, Xiao-Long [1 ]
Gao, Ning [1 ]
Xing, Yan [1 ]
Zhang, Hai-Bin [1 ]
Zhang, Ai-Ling [2 ]
Liu, Jing [1 ]
He, Jin-Long [1 ]
Xu, Yuan [1 ]
Lin, Wen-Mian [3 ]
Chen, Zan-Mou [1 ]
Zhang, Hao [1 ]
Zhang, Zhe [1 ]
Li, Jia-Qi [1 ]
机构：
[1] South China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Natl Engn Res Ctr Breeding Swine Ind, Coll Anim Sci, Guangzhou, Guangdong, Peoples R China
[2] Guangdong Univ Educ, Coll Biol & Food Engn, Guangzhou, Guangdong, Peoples R China
[3] Guangzhou Ribobio Co Ltd, Guangzhou, Guangdong, Peoples R China
来源：
SCIENTIFIC REPORTS | 2016年 / 6卷
基金：
中国国家自然科学基金;
关键词：
GENE BODY METHYLATION; CPG ISLANDS; PROVIDE INSIGHT; METHYLOME; EXPRESSION; PROMOTER; SEQ; IDENTIFICATION; PLURIPOTENT; REVEALS;
D O I：
10.1038/srep22138
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Substantial evidence has shown that DNA methylation regulates the initiation of ovarian and sexual maturation. Here, we investigated the genome-wide profile of DNA methylation in porcine ovaries at single-base resolution using reduced representation bisulfite sequencing. The biological variation was minimal among the three ovarian replicates. We found hypermethylation frequently occurred in regions with low gene abundance, while hypomethylation in regions with high gene abundance. The DNA methylation around transcriptional start sites was negatively correlated with their own CpG content. Additionally, the methylation level in the bodies of genes was higher than that in their 5' and 3' flanking regions. The DNA methylation pattern of the low CpG content promoter genes differed obviously from that of the high CpG content promoter genes. The DNA methylation level of the porcine ovary was higher than that of the porcine intestine. Analyses of the genome-wide DNA methylation in porcine ovaries would advance the knowledge and understanding of the porcine ovarian methylome.
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页数：10
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