Simple transformation of the rice false smut fungus Villosiclava virens by electroporation of intact conidia

被引：17
|
作者：
Tanaka, Eiji [1 ]
Kumagawa, Tsuyoshi [1 ]
Tanaka, Chihiro [2 ]
Koga, Hironori [1 ]
机构：
[1] Ishikawa Prefectural Univ, Nonoichi, Ishikawa 9218836, Japan
[2] Kyoto Univ, Grad Sch Agr, Sakyo Ku, Kyoto 6068502, Japan
来源：
MYCOSCIENCE | 2011年 / 52卷 / 05期
关键词：
Hygromycin B; Infection; Rice disease; Secondary conidia; Ustilaginoidea virens; MEDIATED TRANSFORMATION; USTILAGINOIDEA-VIRENS; PATHOGEN; DNA;
D O I：
10.1007/s10267-011-0115-6
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A simple procedure is reported for transformation of the rice false smut fungus Villosiclava virens (anamorph: Ustilaginoidea virens) using electroporation of intact conidial cells. The transformation vector pCB1004eGFP was constructed with a green fluorescent protein (eGFP) gene under a constitutive promoter of the glyceraldehyde-3-phosphate dehydrogenase gene of Cochliobolus heterostrophus. When a linearized vector was applied, eGFP-expressing transformants were successfully acquired. An inoculation test in rice plants showed that the eGFP-expressing transformants were able to form rice false smut balls.
引用
收藏
页码：344 / 348
页数：5
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