Statistical data analysis of bacterial t-RFLP profiles in forensic soil comparisons

被引：33
|
作者：
Quaak, Frederike C. A. [1 ]
Kuiper, Irene [1 ]
机构：
[1] Netherlands Forens Inst, NL-2497 GB The Hague, Netherlands
来源：
FORENSIC SCIENCE INTERNATIONAL | 2011年 / 210卷 / 1-3期
关键词：
Forensic science; Soil; Bacteria; DNA; t-RFLP; Bray-Curtis distance; 16S RIBOSOMAL-RNA; FRAGMENT-LENGTH-POLYMORPHISMS; MICROBIAL COMMUNITIES; RESTRICTION; ORDINATION; GENES; SEDIMENT; DGGE;
D O I：
10.1016/j.forsciint.2011.02.005
中图分类号：
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号：
0301 ; 10 ;
摘要：
Soil can play an important role in forensic investigations in linking suspects or objects to a crime scene. Bacterial populations are one of the biotic parameters in soil which can be used for comparisons. Terminal restriction fragment length polymorphism (t-RFLP) is used to visualize these populations. Here we present a method to compare soil t-RFLP profiles based on Bray-Curtis distances. We developed a decision model to predict the possible common source of unknown samples. Test cases in cooperation with the Police Academy of the Netherlands were used to validate the decision model. The results of these test cases are very promising, indicating that bacterial profiling is a useful additional tool in forensic soil comparisons. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：96 / 101
页数：6
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