A membrane fusion assay based on pore-spanning lipid bilayers

被引：11
|
作者：
Hoefer, Ines [1 ]
Steinem, Claudia [1 ]
机构：
[1] Inst Organ & Biomol Chem, D-37077 Gottingen, Germany
来源：
SOFT MATTER | 2011年 / 7卷 / 05期
关键词：
SUSPENDING MEMBRANES; IMPEDANCE ANALYSIS; INFLUENZA-VIRUS; SINGLE-VESICLE; MICRO-BLMS; HEMAGGLUTININ; HEMIFUSION; DOCKING; DRIVEN;
D O I：
10.1039/c0sm01429j
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
Pore-spanning planar membranes on highly ordered porous silicon substrates were shown to be well suited to monitor the calcium ion mediated fusion of large unilamellar vesicles by means of confocal laser scanning fluorescence microscopy and scanning ion conductance microscopy in real-time.
引用
收藏
页码：1644 / 1647
页数：4
相关论文
共 50 条
  • [31] Pore Spanning Lipid Bilayers on Mesoporous Silica Having Varying Pore Size
    Claesson, Maria
    Frost, Rickard
    Svedhem, Sofia
    Andersson, Martin
    [J]. LANGMUIR, 2011, 27 (14) : 8974 - 8982
  • [32] Pore spanning lipid bilayers on silanised nanoporous alumina membranes
    Jani, Abdul Mutalib Md
    Zhou, Jinwen
    Nussio, Matthew R.
    Losic, Dusan
    Shapter, Joe G.
    Voelcker, Nicolas H.
    [J]. SMART MATERIALS V, 2008, 7267
  • [33] Planar Pore-Spanning Membranes: A Platform to Study Snare-Mediated Fusion Processes
    Hubrich, Raphael
    Kuhlmann, Jan
    Schwenen, Lando L. G.
    Milovanovic, Dragomir
    Jahn, Reinhard
    Geil, Burkhard
    Steinem, Claudia
    [J]. BIOPHYSICAL JOURNAL, 2016, 110 (03) : 520A - 520A
  • [34] PORE-SPANNING PPY(DBS) AS A VOLTAGE-GATED SYNTHETIC MEMBRANE ION CHANNEL
    Hery, Travis M.
    Sundaresan, Vishnu-Baba
    [J]. PROCEEDINGS OF THE ASME CONFERENCE ON SMART MATERIALS, ADAPTIVE STRUCTURES AND INTELLIGENT SYSTEMS, 2016, VOL 2, 2016,
  • [35] Pore-spanning membranes: a tool to study single vesicle content release in SNARE driven fusion
    Muehlenbrock, P.
    Hubrich, R.
    Kuhlmann, J.
    Schwenen, L.
    [J]. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 2017, 46 : S307 - S307
  • [36] Pulling Tethers from Pore-Spanning Bilayers: Towards Simultaneous Determination of Local Bending Modulus and Lateral Tension of Membranes
    Kocun, Marta
    Janshoff, Andreas
    [J]. SMALL, 2012, 8 (06) : 847 - 851
  • [37] In situ generation of electrochemical gradients across pore-spanning membranes
    Frese, Daniel
    Steltenkamp, Siegfried
    Schmitz, Sam
    Steinem, Claudia
    [J]. RSC ADVANCES, 2013, 3 (36) : 15752 - 15761
  • [38] Formation of Nanopore-Spanning Lipid Bilayers through Liposome Fusion
    Kumar, Karthik
    Isa, Lucio
    Egner, Alexander
    Schmidt, Roman
    Textor, Marcus
    Reimhult, Erik
    [J]. LANGMUIR, 2011, 27 (17) : 10920 - 10928
  • [39] Indentation of pore-spanning lipid membranes: Spring-stiffening or-softening responses and apparent stiffness prediction
    Zhang, Xinyun
    Shi, Chao
    Wu, Zemimg
    Yi, Xin
    [J]. EXTREME MECHANICS LETTERS, 2022, 57
  • [40] Viscoelasticity of pore-spanning polymer membranes derived from giant polymersomes
    Kocun, Marta
    Mueller, Waltraut
    Maskos, Michael
    Mey, Ingo
    Geil, Burkhard
    Steinem, Claudia
    Janshoff, Andreas
    [J]. SOFT MATTER, 2010, 6 (11) : 2508 - 2516
12345