Streptomyces indicus sp nov., an actinomycete isolated from deep-sea sediment

The taxonomic position of an actinomycete isolated from deep-sea sediment from the Indian Ocean was determined by using a polyphasic approach. The presence of iso-C(15:0), anteiso-C(15:0), iSO-C(16:0), iSO-C(17:0) and anteiso-C(17:0) as the major cellular fatty acids, LL-diaminopimelic acid as the characteristic diamino acid, and MK-9(H(4), H(6) and H(8)) as the major menaquinones supported the affiliation of strain IH32-1(T) to the genus Streptomyces. Comparison of 16S rRNA gene sequences showed that strain IH32-1(T) exhibited highest similarities to the type strains of Streptomyces globosus (97.6%) and Streptomyces toxytricini (97.6%). However, DNA-DNA relatedness values between strain IH32-1(T) and the type strains of S. globosus and S. toxytricini were determined as 55.2 +/- 4.7 and 38.3 +/- 2.5%, respectively. Based on its chemotaxonomic, phenotypic and genotypic characteristics, strain IH32-1(T) is considered to represent a novel species in the genus Streptomyces, for which the name Streptomyces indicus sp. nov. is proposed. The type strain is IH32-1(T) (=DSM 42001(T)=CGMCC 4.5727(T)).