Effects of metal ions on plasma membrane Mg2+-ATPase in rat uterus and ovaries

被引：4
|
作者：
Milosevic, M [1 ]
Petrovic, S [1 ]
Demajo, M [1 ]
Horvat, A [1 ]
机构：
[1] Inst Nucl Sci Vinca, Lab Mol Biol & Endocrinol, Belgrade 11001, Serbia Monteneg
来源：
BIOPHYSICS FROM MOLECULES TO BRAIN: IN MEMORY OF RADOSLAV K. ANDJUS | 2005年 / 1048卷
关键词：
Mg2+-ATPase; ecto-ATPase; cadmium; mercury; ovary; uterus; rat;
D O I：
10.1196/annals.1342.061
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
The in vitro effects of cadmium and mercury were investigated on the Mg2+-ATPase activity of plasma membranes from the rat ovary and uterus. ATP hydrolyzing activities were significant and dose-dependent-inhibited in both plasma membrane preparations by both metals. According to the IC50 and apparent K-i, Cd2+ was most potent in the ovary, while Hg2+ was most potent in the uterus. In ovaries and uterus,Cd2+ inhibits competitively, while Hg2+ inhibits noncompetitively in both organs. The observed inhibition was a consequence of direct action of the chosen metal ions on the enzyme protein and by decreasing ATP hydrolysis, Hg2+ and Cd2+ may affect mammalian fertility.
引用
收藏
页码：445 / 448
页数：4
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