INTERACTION OF GOLD NANOPARTICLES WITH RAT BRAIN SYNAPTOSOMAL PLASMA MEMBRANE Na+/K+ - ATPase AND Mg2+-ATPase

被引：0

作者：

Petrovic, Voin ^{[1
]}

Vodnik, Vesna ^{[1
]}

Stanojevic, Ivana ^{[1
]}

Rakocevic, Zlatko ^{[1
]}

Vasic, Vesna ^{[1
]}

机构：

[1] Univ Belgrade, Vinca Inst Nucl Sci, Belgrade, Serbia

来源：

DIGEST JOURNAL OF NANOMATERIALS AND BIOSTRUCTURES | 2012年 / 7卷 / 02期

关键词：

Gold nanoparticles; Na+/K+-ATPase; Mg2+-ATPase; synaptosomal plasma membranes; biofunctionalization; DEPENDENT CYTOTOXICITY; NA; K-ATPASE; INHIBITION; BINDING; SIZE; CYSTEINE; DIGOXIN; PROTEIN; ENZYME;

D O I：

暂无

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

The aim of the work was to investigate the interaction between borate capped gold nanoparticles (NPs) and the rat brain synaptosomal plasma membranes (SPM), as well as the effects of these NPs on SPM Na+/K+ - ATPase and Mg2+-ATPase activity. The changes in the UV-vis spectra of NPs and SPM assembly suggested the agglomeration and precipitation of NPs. FTIR measurements indicated that both protein -SH and -NH2 groups and positively charged membrane fragments were implicated in the adhesion of SPM to the surface of NPs. AFM showed an increase in the particularization of the SPM material after mixing with gold NPs. Influence of gold NPs on Na+/K+-ATPase and Mg2+-ATPase activity was investigated as the function of NPs and protein concentration, preincubation time and also in the presence of various concentrations of ouabain, the specific enzyme inhibitor. NPs induced the stimulation of Na+/K+-ATPase activity for more than 100%, since Mg2+-ATPase activity reminded unaffected. We propose that this stimulation of enzyme activity was a consequence of an increase of the active surface of membranes.

引用

页码：423 / 433

页数：11

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