Sonoporation of endothelial cells by vibrating targeted microbubbles

被引:137
|
作者
Kooiman, Klazina [1 ]
Foppen-Harteveld, Miranda [1 ]
van der Steen, Antonius F. W. [1 ,2 ]
de Jong, Nico [1 ,2 ,3 ]
机构
[1] Erasmus MC, Dept Biomed Engn, Thoraxctr, NL-3000 CA Rotterdam, Netherlands
[2] Interuniv Cardiol Inst Netherlands, Utrecht, Netherlands
[3] Univ Twente, Phys Fluids Grp, NL-7500 AE Enschede, Netherlands
关键词
Drug delivery; Ultrasound; Targeted microbubble; Sonoporation; Endothelial cells; ULTRASOUND CONTRAST AGENTS; ADHESION MOLECULE-1; DRUG-DELIVERY; ACOUSTIC CAVITATION; INERTIAL CAVITATION; TUMOR ANGIOGENESIS; PLASMA-MEMBRANE; P-SELECTIN; PECAM-1; ECHOCARDIOGRAPHY;
D O I
10.1016/j.jconrel.2011.04.008
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Molecular imaging using ultrasound makes use of targeted microbubbles. In this study we investigated whether these microbubbles could also be used to induce sonoporation in endothelial cells. Lipid-coated microbubbles were targeted to CD31 and insonified at 1 MHz at low peak negative acoustic pressures at six sequences of 10 cycle sine-wave bursts. Vibration of the targeted microbubbles was recorded with the Brandaris-128 high-speed camera (similar to 13 million frames per second). In total, 31 cells were studied that all had one microbubble (1.2-4.2 micron in diameter) attached per cell. After insonification at 80 kPa, 30% of the cells (n = 6) had taken up propidium iodide, while this was 20% (n = 1) at 120 kPa and 83% (n = 5) at 200 kPa. Irrespective of the peak negative acoustic pressure, uptake of propidium iodide was observed when the relative vibration amplitude of targeted microbubbles was greater than 0.5. No relationship was found between the position of the microbubble on the cell and induction of sonoporation. This study shows that targeted microbubbles can also be used to induce sonoporation, thus making it possible to combine molecular imaging and drug delivery. (c) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:35 / 41
页数:7
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