Expression of Peroxiredoxins and Pulmonary Surfactant Protein A Induced by Silica in Rat Lung Tissue

被引：9

作者：

Liu Nan ^{[1
]}

Xue Ling ^{[1
]}

Guan Yi ^{[1
]}

Li Qing Zhao ^{[1
]}

Cao Fu Yuan ^{[1
]}

Pang Shu Lan ^{[1
]}

Guan Wei Jun ^{[1
]}

机构：

[1] North China Univ Sci & Technol, Sch Publ Hlth, Hebei Prov Coal Mine Hlth & Safety Lab, Tangshan 063000, Hebei, Peoples R China

来源：

BIOMEDICAL AND ENVIRONMENTAL SCIENCES | 2016年 / 29卷 / 08期

关键词：

D O I：

10.3967/bes2016.077

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Silicosis is one of the most serious occupational diseases in China and dates back to centuries ago. In this study, we successfully established a rat model of silicosis by intratracheal silica injection for 28 days and determined hydroxyproline levels to evaluate collagen metabolism in lung homogenates. Oxidative stress status was evaluated by detecting catalase and glutathione peroxidase activities. Expression levels of peroxiredoxins (Prx I and Prx VI) were detected by Western blotting. Pulmonary surfactant protein A (SP-A) levels in rat serum and lung tissue were analyzed by ELISA, and SP-A and Prx expression levels in lung tissues were detected by immunohistochemistry. The results suggest that Prx proteins may be involved in pulmonary fibrosis induced by silica. Downregulation of SP-A expression caused due to silica is an important factor in the occurrence and development of silicosis.

引用

页码：584 / 588

页数：5

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