Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells

被引：281
|
作者：
Hoeck, Julia
Weinmann, Lasse
Ender, Christine
Ruedel, Sabine
Kremmer, Elisabeth
Raabe, Monika
Urlaub, Henning
Meister, Gunter [1 ]
机构：
[1] Max Planck Inst Biochem, Lab RNA Biol, Martinsried, Germany
[2] GSF Munich, Inst Mol Immunol, Munich, Germany
[3] Max Planck Inst Biophys Chem, Bioanalyt Mass Spectrometry Grp, Gottingen, Germany
来源：
EMBO REPORTS | 2007年 / 8卷 / 11期
关键词：
Argonaute proteins; gene silencing; microRNA; RNA interference;
D O I：
10.1038/sj.embor.7401088
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Members of the Argonaute ( Ago) protein family associate with small RNAs and have important roles in RNA silencing. Here, we analysed Ago1- and Ago2- containing protein complexes in human cells. Separation of Ago- associated messenger ribonucleoproteins ( mRNPs) showed that Ago1 and Ago2 reside in three complexes with distinct Dicer and RNA- induced silencing complex activities. A comprehensive proteomic analysis of Ago- containing mRNPs identified a large number of proteins involved in RNA metabolism. By using co- immunoprecipitation experiments followed by RNase treatment, we biochemically mapped interactions within Ago mRNPs. Using reporter assays and knockdown experiments, we showed that the putative RNA- binding protein RBM4 is required for microRNA- guided gene regulation.
引用
收藏
页码：1052 / 1060
页数：9
相关论文
共 50 条
  • [41] Computational identification of biologically functional non-hairpin GC-helices in human Argonaute mRNA
    Simon Dornseifer
    Georg Sczakiel
    BMC Bioinformatics, 14
  • [42] Computational identification of biologically functional non-hairpin GC-helices in human Argonaute mRNA
    Dornseifer, Simon
    Sczakiel, Georg
    BMC BIOINFORMATICS, 2013, 14
  • [43] Proteomic analysis of SUMOylated protein in mammalian cells
    Liu, Xiaoyan
    Zhang, Fujian
    Zhai, Jianjun
    Zhu, Haining
    FASEB JOURNAL, 2008, 22
  • [44] Proteomic analysis of complexes formed by human topoisomerase I
    Czubaty, A
    Girstun, A
    Kowalska-Loth, B
    Trzcinska, AA
    Purta, E
    Winczura, A
    Grajkowski, W
    Staron, K
    BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2005, 1749 (01): : 133 - 141
  • [45] Towards comprehensive proteomic analysis of complexes, organelles and cells
    Yates, JR
    FASEB JOURNAL, 2005, 19 (04): : A251 - A251
  • [46] Towards comprehensive proteomic analysis of complexes, organelles and cells
    Yates, John R.
    Wu, Christine
    MacCoss, Michael
    Venable, John
    Sadygov, Rovshan
    Anderson, Scott
    MOLECULAR & CELLULAR PROTEOMICS, 2004, 3 (10) : S209 - S209
  • [47] Molecular crowding and conserved interface interactions of human argonaute protein-miRNA-target mRNA complex
    Chitara, Dheeraj
    Anand, Richa
    Sanjeev, B. S.
    JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 2021, 39 (17): : 6370 - 6383
  • [48] Analysis of human milk protein complexes containing biologically active proteins and peptides
    Baklanova, A.
    Grishina, T.
    Krasovskaya, I.
    Romanovskaya, E.
    Tsvetkova, E.
    Stefanov, V.
    Leonova, L.
    FEBS OPEN BIO, 2021, 11 : 194 - 195
  • [49] Systems-wide Proteomic Analysis in Mammalian Cells Reveals Conserved, Functional Protein Turnover
    Cambridge, Sidney B.
    Gnad, Florian
    Chuong Nguyen
    Bermejo, Justo Lorenzo
    Krueger, Marcus
    Mann, Matthias
    JOURNAL OF PROTEOME RESEARCH, 2011, 10 (12) : 5275 - 5284
  • [50] Proteomic analysis of NMDA receptor–adhesion protein signaling complexes
    Holger Husi
    Malcolm A. Ward
    Jyoti S. Choudhary
    Walter P. Blackstock
    Seth G. N. Grant
    Nature Neuroscience, 2000, 3 : 661 - 669
12345