Development of microsatellite markers for the endangered orchid Calanthe izu-insularis (Orchidaceae)

被引：4
|
作者：
Nakahama, Naoyuki [1 ,2 ]
Ito, Azusa [2 ]
Kaneko, Shingo [2 ,3 ]
Matsuki, Yu [4 ]
Suyama, Yoshihisa [4 ]
Hayano, Azusa [5 ,6 ]
Murayama, Miho [6 ]
Isagi, Yuji [2 ]
机构：
[1] Univ Tokyo, Grad Sch Arts & Sci, Meguro Ku, 3-8-1 Komaba, Tokyo 1538902, Japan
[2] Kyoto Univ, Grad Sch Agr, Sakyo Ku, Kitashirakawa Oiwake Cho, Kyoto 6068502, Japan
[3] Fukushima Univ, Div Environm Syst Management, 1 Kanayagawa, Fukushima 9601296, Japan
[4] Tohoku Univ, Grad Sch Agr Sci, Kawatabi Field Sci Ctr, 232-3 Yomogida, Osaki, Miyagi 9896711, Japan
[5] Kyoto Prefectural Univ Med, Grad Sch Hlth Care Sci, Kamigyo Ku, Kajii Cho, Kyoto 6028566, Japan
[6] Kyoto Univ, Wildlife Res Ctr, Sakyo Ku, 2-24 Tanaka Sekiden Cho, Kyoto 6068203, Japan
来源：
GENES & GENETIC SYSTEMS | 2018年 / 93卷 / 01期
关键词：
Calanthe; conservation; endangered orchid; natural hybridization; next-generation sequencing;
D O I：
10.1266/ggs.17-00021
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Microsatellite markers were developed for the endangered orchid Calanthe izu-insularis (Orchidaceae). This species is unique to the Izu Islands in Japan. Unfortunately, its population size has decreased because of excessive collection for horticultural purposes. In addition, although natural hybridization between C. izu-insularis and C. discolor var. discolor has been reported, morphological differences between C. izu-insularis and the hybridized individuals remain unclear. Using next-generation sequencing, 11 polymorphic microsatellite markers were developed. All developed markers could amplify C. aristulifera and nine markers could amplify C. d. var. discolor, two other orchid species that are also endangered in Japan. The number of alleles and expected heterozygosity at each locus were 1-6 (mean, 2.35) and 0.00-0.79 (mean, 0.30), respectively. These microsatellite markers will help conservation geneticists in their investigation of the proportion of pure C. izu-insularis individuals in the Izu Islands.
引用
收藏
页码：31 / 35
页数：5
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