Establishment of a human fibroblast cell line producing tumor necrosis factor α (KMST-6/TNF) and growth inhibitory effects of its conditioned medium on malignant cells in culture

被引：0
|
作者：
Fushimi, K
Torigoe, K
Yamauchi, H
Furusako, S
Kurimoto, M
Namba, M
机构：
[1] Okayama Univ, Sch Med, Inst Mol & Cellular Biol, Dept Cell Biol, Okayama 700, Japan
[2] Hayashibara Biochem Labs Inc, Fujisaki Inst, Okayama 702, Japan
来源：
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL | 1998年 / 34卷 / 06期
关键词：
gene therapy; TNF-alpha; cancer cells; human;
D O I：
暂无
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
To develop a new gene therapy model for cancer, a clonal cell line (KMST-6/TNF) which produces human tumor necrosis factor alpha (hTNF-alpha) has been developed by introducing hTNF-alpha cDNA into a human immortal fibroblast cell line (KMST-6). The conditioned medium (CM) of KMST-6/TNF cells inhibited the growth of various malignant human cell lines, but not that of normal human fibroblasts. Although the growth inhibitory effects of KMST-6/TNF CM were neutralized to a considerable degree by anti-TNF-alpha antibody, its inhibitory effects were more marked than the purified human natural TNF-alpha itself in the same units, suggesting that KMST-6/TNF CM contains some growth inhibitory substances other than TNF-alpha. However, interferons alpha, beta, and gamma were undetectable in the KMST-6/TMF CM.
引用
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页码：463 / 467
页数：5
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