Synthesis of 5′-Thio-3′-O-ribonucleoside Phosphoramidites

被引：3

作者：

Li, Nan-Sheng ^{[1
]}

Lu, Jun

Piccirilli, Joseph A. ^{[1
]}

机构：

[1] Univ Chicago, Dept Biochem & Mol Biol, 929 East 57th St, Chicago, IL 60637 USA

来源：

JOURNAL OF ORGANIC CHEMISTRY | 2017年 / 82卷 / 23期

关键词：

SOLID-PHASE SYNTHESIS; RIBOZYME-MEDIATED CLEAVAGE; GENERAL ACID CATALYSIS; HAMMERHEAD RIBOZYME; BRIDGING; 5-PHOSPHOROTHIOATE; DNA TOPOISOMERASE; SUBSTRATE-ANALOG; BASE CATALYSIS; RNA; MECHANISM;

D O I：

10.1021/acs.joc.7b01484

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The chemical synthesis of phosphoramidite derivatives of all four 5'-deoxy-5'-thioribonucleosides is described. These phosphoramidites contained trityl (A, G, C, and U), dimethoxytrityl (A and G), or tert-butyldisulfanyl (G) as the 5'-S-protecting group. The application of several of these phosphoramidites for solid-phase synthesis of oligoribonucleotides containing a 2'-O-photocaged 5'-S-phosphorothiolate linkage or 5'-thiol-labeled RNAs is also further investigated.

引用

页码：12003 / 12013

页数：11

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