Expression of the gene for homoserine kinase of Escherichia coli in mammalian cells

被引：0

作者：

Abramyan, LG ^{[1
]}

Tsymbalyuk, ES ^{[1
]}

Gazaryan, TG ^{[1
]}

Levchenko, IV ^{[1
]}

Andreeva, NB ^{[1
]}

Gazaryan, KG ^{[1
]}

机构：

[1] RUSSIAN ACAD SCI,FRUMKIN INST ELECTROCHEM,MOSCOW 117071,RUSSIA

来源：

MOLECULAR BIOLOGY | 1996年 / 30卷 / 04期

关键词：

homoserine; homoserine kinase; electroporation;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The thrB gene of E. coli coding for homoserine kinase [EC 2.7.1.39] was introduced into the pRc/RSV mammalian expression vector. Transfection of the CHO cell line with the resulting pRSV-Bneo plasmid by electroporation yielded a stably transformed clone accumulating o-homoserine phosphate. Assay for homoserine kinase in cell extracts revealed enzyme activity of 6.0 . 10(-3) units per mg protein, which was 75% that in E. coli cells cultured in the minimal medium and about 20 times that in the 3T3 clones obtained before.

引用

页码：494 / 498

页数：5

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