Expression of the gene for homoserine kinase of Escherichia coli in mammalian cells

被引：0
|
作者：
Abramyan, LG [1 ]
Tsymbalyuk, ES [1 ]
Gazaryan, TG [1 ]
Levchenko, IV [1 ]
Andreeva, NB [1 ]
Gazaryan, KG [1 ]
机构：
[1] RUSSIAN ACAD SCI,FRUMKIN INST ELECTROCHEM,MOSCOW 117071,RUSSIA
来源：
MOLECULAR BIOLOGY | 1996年 / 30卷 / 04期
关键词：
homoserine; homoserine kinase; electroporation;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The thrB gene of E. coli coding for homoserine kinase [EC 2.7.1.39] was introduced into the pRc/RSV mammalian expression vector. Transfection of the CHO cell line with the resulting pRSV-Bneo plasmid by electroporation yielded a stably transformed clone accumulating o-homoserine phosphate. Assay for homoserine kinase in cell extracts revealed enzyme activity of 6.0 . 10(-3) units per mg protein, which was 75% that in E. coli cells cultured in the minimal medium and about 20 times that in the 3T3 clones obtained before.
引用
收藏
页码：494 / 498
页数：5
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