HER2 Gene Amplification by Fluorescence In Situ Hybridization (FISH) Compared With Immunohistochemistry (IHC) in 122 Equivocal Gastric Cancer Cases

被引：10
|
作者：
Liu, Xiaoyan [1 ]
Wang, Xiaoling [1 ]
Wang, Bo [1 ]
Ren, Guoping [1 ]
Ding, Wei [1 ]
机构：
[1] Zhejiang Univ, Coll Med, Affiliated Hosp 1, Dept Pathol, 79 Qingchun Rd, Hangzhou 310003, Zhejiang, Peoples R China
来源：
APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY | 2016年 / 24卷 / 07期
关键词：
gastric cancer; fluorescence in situ hybridization; HER2/neu; PROTEIN EXPRESSION; BREAST-CANCER; NEU ONCOGENE; RECEPTOR; ADENOCARCINOMA; VALIDATION; PROGNOSIS; CARCINOMA; PATTERNS;
D O I：
10.1097/PAI.0000000000000219
中图分类号：
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号：
100101 ;
摘要：
Objective: To determine the frequency of HER2/neu amplification and the relationship between HER2/neu expression and clinicopathologic features in gastric cancers scored immunohistochemistry (IHC) 2+. Methods: A total of 122 gastric cancer cases scored IHC 2+ were retrospectively analyzed for HER2 amplification by fluorescence in situ hybridization (FISH). The correlation between HER2/neu expression and clinicopathologic features was analyzed. Results: HER2/neu gene was amplified in 17 out of the 122 gastric cancer samples. The concordance rate between IHC and FISH was 13.9%. HER2/neu status was correlated with the age of patients (P<0.05). Polysomy of CEP17 was demonstrated in 46 cases, 11 of which (23.91%) were amplified for HER2/neu. Within the subgroups, a correlation between CEP17 polysomy and the depth of invasion was observed (P<0.05). Conclusions: The results highlight the necessity of FISH test for further categorization when gastric cancer cases are scored 2+ by IHC. The value of HER2/neu for a potential role as a negative prognostic factor in the equivocal gastric cancer cases is limited.
引用
收藏
页码：459 / 464
页数：6
相关论文
共 50 条
  • [31] Comparative Assessment of Immunohistochemistry (IHC), Fluorescence In Situ Hybridization (FISH), and Silver-Enhanced In Situ Hybridization (SISH) for Evaluation of HER2 Gene Status in Breast Carcinoma
    Ryu, Y.
    Jeong, H.
    Kim, Y.
    Kim, B.
    Kim, H.
    Kim, A.
    LABORATORY INVESTIGATION, 2010, 90 : 70A - 70A
  • [32] Comparative Assessment of Immunohistochemistry (IHC), Fluorescence In Situ Hybridization (FISH), and Silver-Enhanced In Situ Hybridization (SISH) for Evaluation of HER2 Gene Status in Breast Carcinoma
    Ryu, Y.
    Jeong, H.
    Kim, Y.
    Kim, B.
    Kim, H.
    Kim, A.
    MODERN PATHOLOGY, 2010, 23 : 70A - 70A
  • [33] Comparison of HER2 expression by immunohistochemistry (IHC) using automated imaging system and fluorescence in situ hybridization (FISH). A retrospective analysis of 2853 cases
    Collins, R.
    Xiang, D.
    Christie, A.
    Leitch, M.
    Euhus, D.
    Rao, R.
    Haley, B.
    Sarode, V.
    CANCER RESEARCH, 2012, 72
  • [34] Comparison of immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) assessment for Her-2 status in breast cancer
    Weiguo Sui
    Minglin Ou
    Jiejing Chen
    Youhua Wan
    Hongbo Peng
    Minfang Qi
    He Huang
    Yong Dai
    World Journal of Surgical Oncology, 7
  • [35] Comparison of immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) assessment for Her-2 status in breast cancer
    Sui, Weiguo
    Ou, Minglin
    Chen, Jiejing
    Wan, Youhua
    Peng, Hongbo
    Qi, Minfang
    Huang, He
    Dai, Yong
    WORLD JOURNAL OF SURGICAL ONCOLOGY, 2009, 7 : 83
  • [36] HER2 expression in gastric cancer in Indian population—An immunohistochemistry and fluorescence in situ hybridization study
    Anuradha Sekaran
    Raja Sekhar Kandagaddala
    Santosh Darisetty
    Sandeep Lakhtakia
    Santa Ayyagari
    Guduru Venkat Rao
    Pradeep Rebala
    Duvvuru Bhaskara Reddy
    Duvvuru Nageshwar Reddy
    Indian Journal of Gastroenterology, 2012, 31 (3) : 106 - 110
  • [37] HER-2/neu overexpression:: immunohistochemistry (IHC) or fluorescence in-situ hybridization (FISH)?
    Bunis, JA
    Weaver, DL
    Wells, WA
    Cole, BF
    Memoli, V
    Cates, J
    Schwartz, G
    Muss, HB
    Kaufman, PA
    BREAST CANCER RESEARCH AND TREATMENT, 2003, 82 : S142 - S142
  • [38] Concomitant Detection of HER2 Protein and Gene Alterations by Immunohistochemistry (IHC) and Silver Enhanced In Situ Hybridization (SISH) Identifies HER2 Positive Breast Cancer with and without Gene Amplification
    Varga, Zsuzsanna
    Tubbs, Raymond R.
    Moch, Holger
    PLOS ONE, 2014, 9 (08):
  • [39] A fluorescence in situ hybridization (FISH) microfluidic platform for detection of HER2 amplification in cancer cells
    Kao, Kai-Jie
    Tai, Chien-Hsuan
    Chang, Wen-Hsin
    Yeh, Ta-Sen
    Chen, Tse-Ching
    Lee, Gwo-Bin
    BIOSENSORS & BIOELECTRONICS, 2015, 69 : 272 - 279
  • [40] HER2 status of gastric and gastroesophageal cancer (GC) patients assessed by immunohistochemistry (IHC), fluorescence in situ hybridization (FISH), and high-resolution array comparative genomic hybridization (aCGH)
    Janjigian, Y. Y.
    Barbashina, V. V.
    Kelsen, D. P.
    Tang, L. H.
    Ilson, D.
    Tafe, L. J.
    Hicks, J. B.
    Shah, M. A.
    JOURNAL OF CLINICAL ONCOLOGY, 2010, 28 (15)
12345