Analysis of Transcriptional Activity of the Arabidopsis thaliana Ornithine-Delta-Aminotransferase Gene Promoter

被引:1
|
作者
Gerasimova, S. V. [1 ,2 ]
Gorelova, V. V. [1 ,2 ]
Dorogina, O. V. [3 ]
Zhmud, E. V. [3 ]
Koval, V. S. [1 ,2 ]
Romanova, A. V. [1 ,2 ]
Kochetova, A. V. [1 ,2 ]
Shumny, V. K. [1 ,2 ]
机构
[1] Russian Acad Sci, Inst Cytol & Genet, Novosibirsk 630090, Russia
[2] Novosibirsk State Univ, Dept Cytol & Genet, Novosibirsk 630090, Russia
[3] Russian Acad Sci, Cent Siberian Bot Garden, Novosibirsk 630090, Russia
基金
俄罗斯基础研究基金会;
关键词
PROLINE BIOSYNTHESIS; EXPRESSION; VECTORS; STRESS;
D O I
10.1134/S1022795411050073
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Cloning of the Arabidopsis thaliana genomic DNA fragment presumably corresponding to the promoter region of the ornithine-delta-aminotransferase (OAT) gene is reported. The reporter-gene construct, containing the Escherichia coli beta-glucouronidase gene under control of the OAT gene promoter was generated. The Nicotian tabacum SR1 transformants carrying this construct were obtained. It was demonstrated that under normal conditions, expression of the reporter gene was associated with the meristems and the zones of intensive shoot growth. Possible role of the OAT gene in nitrogen metabolism and shoot development is discussed.
引用
收藏
页码:625 / 628
页数:4
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