Dynamic expression and cellular localization of the Drosophila 14-3-3ε during embryonic development

The 14-3-3 protein family is known to interact with various proteins involved in signaling pathways. We report here the expression pattern of the Drosophila 14-3-3 (d14-3-3 epsilon) protein during embryonic development. in syncytial blastoderm when the nuclei divided rapidly, d14-3-3 epsilon localized in the nuclei. During cellularization d14-3-3 epsilon gradually became membrane-bound. During gastrulation, an enhanced staining in the perinuclear region was observed in various tissues. Co-labeling with dp-ERK which recognized the activated form of MAPK suggested that d14-3-3 epsilon was expressed prior to MAPK activation. During neuronal differentiation, the d14-3-3 epsilon protein remained at a high level in the neuronal cytoplasm. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.