Analysis of methylene blue and its metabolites in blood by capillary electrophoresis/electrospray ionization mass spectrometry

被引：6
|
作者：
Yang, Fang [1 ,2 ]
Xia, Shifei [1 ]
Liu, Zhencai [2 ]
Chen, Jian [2 ]
Lin, Yonghui [2 ]
Qiu, Bin [1 ]
Chen, Guonan [1 ]
机构：
[1] Fuzhou Univ, Dept Chem, Minist Educ, Key Lab Anal & Detect Technol Food Safety, Fuzhou 350002, Fujian, Peoples R China
[2] Fujian Entry Exit Inspect & Quarantine Bur, Fuzhou, Fujian, Peoples R China
来源：
ELECTROPHORESIS | 2011年 / 32卷 / 6-7期
关键词：
CE-ESI-MS; Metabolites; Methylene blue; Rat blood; HIGHLY IONIZED DRUGS; PHARMACOKINETICS; ENCEPHALOPATHY; TISSUE; URINE; COMBINATION; DOG;
D O I：
10.1002/elps.201000514
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A method for the determination of methylene blue (MB) and its metabolites (azure A, azure B and azure C) in rat blood by CE-electrospray ionization mass spectrometry (CE-ESI-MS) was developed in this paper. Different analytical parameters were investigated in detail such as pH and concentration of separation buffer, and ESI-MS instrumental parameters. Under the optimum conditions, MB and its metabolites were separated and detected in 27.3 min. LODs (defined as S/N = 3) of this method were 0.22, 0.25, 0.10 and 0.30 mu g/mL for MB, azure A, azure B and azure C, respectively. To get a satisfactory extraction efficiency of MB and its metabolites in rat blood, different extraction solutions were studied. By using this method, MB and its metabolites (azure A, azure B and azure C) were successfully analyzed in rat blood samples.
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页码：659 / 664
页数：6
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