Susceptibility and mode of binding of the Mycobacterium tuberculosis cysteinyl transferase mycothiol ligase to tRNA synthetase inhibitors

被引:15
|
作者
Gutierrez-Lugo, Maria-Teresa [1 ]
Bewley, Carole A. [1 ]
机构
[1] NIDDK, Bioorgan Chem Lab, NIH, Bethesda, MD 20892 USA
关键词
Mycothiol; Mycobacteria; STD NMR; Antituberculars; Aminoacyl adenosine; GROWTH; ENZYME; MSHC;
D O I
10.1016/j.bmcl.2011.02.042
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
The cysteinyl transferase mycothiol ligase, or MshC, catalyzes the fourth step in the biosynthesis of the small molecular weight thiol mycothiol. MshC is essential for growth of Mycobacterium tuberculosis. Two groups of known aminoacyl tRNA synthetase inhibitors were evaluated for inhibition of M. tuberculosis MshC including aminoacyl adenosine analogs and natural products. Using enzyme assays, isothermal titration calorimetry and NMR, we show that MshC is selectively inhibited by cysteinyl sulfamoyl adenosine, and that discrimination occurs at the amino acid moiety. Published by Elsevier Ltd.
引用
收藏
页码:2480 / 2483
页数:4
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