Distribution of F-actin, α-actinin, tropomyosin, tubulin and organelles in Euglena gracilis by immunofluorescence microscopy

Euglena gracilis, a unicellular flagellated alga, can display numerous shape changes. These changes are most probably caused by a pellicle and an internal cytoskeleton, In this paper we studied the distribution of the cytoskeletal proteins actin, alpha-actinin, tropomyosin and tubulin in dark-adapted Euglena, using immunofluorescence microscopy. We found that F-actin, alpha-actinin, tropomyosin and tubulin have a distribution that is coincident in the plasma membrane and, in addition, alpha-actinin and tropomyosin are seen in small patches in the cytoplasm, and tubulin in the flagella, We have also studied the distribution of the endoplasmic reticulum, nucleus, and Golgi apparatus of these cells, using fluorescent probes. Both the endoplasmic reticulum and the Golgi apparatus have a meshwork pattern distributed throughout the cytoplasm, and the nucleus has a chromatin evenly distributed in the nucleoplasm.