The amino acid sequence of wood duck lysozyme

被引：9
|
作者：
Araki, T [1 ]
Torikata, T [1 ]
机构：
[1] Kyushu Tokai Univ, Sch Agr, Dept Biochem, Kumamoto 8691404, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1999年 / 63卷 / 01期
关键词：
lysozyme; amino acid sequence; egg white;
D O I：
10.1271/bbb.63.220
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The amino acid sequence of wood duck (Aix sponsa) lysozyme was analyzed. Carboxymethylated lysozyme was digested with trypsin and the resulting peptides were sequenced. The established amino acid sequence had the highest similarity to duck III lysozyme with four amino acid substitutions, and had eighteen amino acid substitutions from chicken lysozyme. The valine at position 75 was newly detected in chicken-type lysozymes. In the active site, Tyr34 and Glu57 were found at subsites F and D, respectively, when compared with chicken lysozyme.
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页码：220 / 222
页数：3
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