Cytotoxicity and cellular uptake of perfluorocarboxylic acids

被引:4
|
作者
Kasuya, Maria Carmelita [1 ]
Hatanaka, Kenichi [1 ]
机构
[1] Univ Tokyo, Inst Ind Sci, Meguro Ku, 4-6-1 Komaba, Tokyo 1538505, Japan
关键词
Perfluorocarboxylic acids; Bioaccumulation; Cytotoxicity; Perfluorooctanoic acid; Persistent organic pollutant; Mouse melanoma B16 cells; MASS-SPECTROMETRY; CARBOXYLIC-ACIDS; FISH;
D O I
10.1016/j.jfluchem.2016.05.006
中图分类号
O61 [无机化学];
学科分类号
070301 ; 081704 ;
摘要
To address the health and environmental concerns associated with perfluorocarboxylic acids, the assessment of cytotoxicity and bioaccumulation of perfluorocarboxylic acids is essential. This study investigated the effect of perfluorocarboxylic acids having various chain lengths on mouse melanoma B16 cells. The extent of cytotoxicity of perfluorohexanoic acid (C6), perfluoroheptanoic acid (C7), perfluorooctanoic acid (C8), perfluorononanoic acid (C9) and perfluorodecanoic acid (C10) within a concentration range of 0.25-1600 mu g/ml was determined. Based on results, the viability of cells was 90% or higher in the presence of C6, C7, C8 at a concentration of up to 200 mu g/ml, indicating that B16 cells are safe in the presence of C6, C7 and C8. On the other hand, moderate cytotoxicity was observed with C9 or C10, even at a relatively low concentration of 25 mu g/ml. When cells were incubated in the presence of the same concentration (100 or 200 mu g/ml) of perfluorocarboxylic acids, the number of live cells decreased as the perfluoroalkyl chain length increased suggesting that long-chained perfluorocarboxylic acids are more cytotoxic than short-chained perfluorocarboxylic acids. The correlation between cellular uptake and perfluoroalkyl chain length was also investigated. The presence of C6 in the cells was not detected probably because of poor uptake. On the other hand, the presence of C7-C10 in the cells was confirmed and quantified by LC ESI MS. Results showed that cellular uptake of long-chained perfluorocarboxylic acids were significantly higher than short-chained perfluorocarboxylic acids. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码:1 / 4
页数:4
相关论文
共 50 条
  • [21] Cellular uptake, intracellular trafficking and cytotoxicity of silver nanoparticles
    Singh, Raman Preet
    Ramarao, Poduri
    TOXICOLOGY LETTERS, 2012, 213 (02) : 249 - 259
  • [22] SYNTHESIS, CELLULAR UPTAKE, AND CYTOTOXICITY OF A THERMALLY RESPONSIVE NANOCAPSULE
    Zhang, Wujie
    Rong, Jianhua
    Wang, Qian
    He, Xiaoming
    PROCEEDINGS OF THE ASME SUMMER BIOENGINEERING CONFERENCE - 2009, PT A AND B, 2009, : 357 - 358
  • [23] PYROLYTIC REACTIONS OF HALOANHYDRIDES OF PERFLUOROCARBOXYLIC ACIDS
    PODSEVALOV, PV
    BILDINOV, IK
    RYCHKOV, ML
    DEEV, LE
    IGUMNOV, SM
    ZHURNAL ORGANICHESKOI KHIMII, 1994, 30 (08): : 1207 - 1211
  • [24] A NEW METHOD FOR THE PREPARATION OF PERFLUOROCARBOXYLIC ACIDS
    HUANG, BN
    HAAS, A
    LIEB, M
    JOURNAL OF FLUORINE CHEMISTRY, 1987, 36 (01) : 49 - 62
  • [25] Adsorption of perfluorocarboxylic acids at the silica surface
    Shafique, Umer
    Dorn, Vivien
    Paschke, Albrecht
    Schueuermann, Gerrit
    CHEMICAL COMMUNICATIONS, 2017, 53 (03) : 589 - 592
  • [26] SYNTHESIS, CYTOTOXICITY AND CELLULAR UPTAKE OF NEW, BRANCHED POLYMER CONJUGATES CONTAINING HYDROPHOBIC AMINO ACIDS OR ARGININE AND METHOTREXATE
    Szabo, R.
    Sebestyen, M.
    Koczan, G.
    Hudecz, F.
    JOURNAL OF PEPTIDE SCIENCE, 2016, 22 : S114 - S114
  • [27] THE USE OF PERFLUOROCARBOXYLIC ACIDS AS SEPARATING AGENTS
    MILLS, GF
    WHETSEL, HB
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1955, 77 (17) : 4690 - 4691
  • [28] ACYLATION OF DIALKYLPHOSPHOROUS ACIDS BY ANHYDRIDES OF PERFLUOROCARBOXYLIC ACIDS - PERFLUOROALKYLACYLPHOSPHITES
    MIRONOV, VF
    OFITSEROV, EN
    KONOVALOVA, IV
    BURNAEVA, LA
    ZHURNAL OBSHCHEI KHIMII, 1994, 64 (01): : 69 - 73
  • [29] OXIDATIVE DECARBOXYLATION OF ALIPHATIC PERFLUOROCARBOXYLIC ACIDS
    SERGUCHEV, YA
    DAVYDOVA, VG
    ZHURNAL ORGANICHESKOI KHIMII, 1982, 18 (12): : 2610 - 2611
  • [30] Synthesis of technically useful perfluorocarboxylic acids
    Wroblewska, A
    Meissner, E
    Milchert, E
    JOURNAL OF FLUORINE CHEMISTRY, 2006, 127 (03) : 345 - 350