One-pot enzymatic production of 2-acetamido-2-deoxy-D-galactose (GalNAc) from 2-acetamido-2-deoxy-D-glucose (GlcNAc)

被引：7
|
作者：
Inoue, Kousuke [1 ]
Nishimoto, Mamoru [1 ]
Kitaoka, Motomitsu [1 ]
机构：
[1] Natl Agr & Food Res Org, Natl Food Res Inst, Tsukuba, Ibaraki 3058642, Japan
来源：
CARBOHYDRATE RESEARCH | 2011年 / 346卷 / 15期
关键词：
2-Acetamido-2-deoxy-D-galactose; 2-Acetamido-2-deoxy-D-glucose; One-pot enzymatic reaction; Bifidobacterium; Galacto-N-biose/lacto-N-biose I pathway; N-Acetylhexosamine; 1-kinase; GLYCOSIDE HYDROLASE FAMILY; N-BIOSE-I; BIFIDOBACTERIUM-BIFIDUM; SUBSTRATE-SPECIFICITY; MILK OLIGOSACCHARIDES; MOLECULAR-CLONING; IDENTIFICATION; DEGRADATION; PATHWAY; LONGUM;
D O I：
10.1016/j.carres.2011.08.032
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
2-Acetamido-2-deoxy-D-galactose (GalNAc) is a common monosaccharide found in biologically functional sugar chains, but its availability is often limited due to the lack of abundant natural sources. In order to produce GalNAc from abundantly available sugars, 2-acetamido-2-deoxy-D-glucose (GlcNAc) was converted to GalNAc by a one-pot reaction using three enzymes involved in the galacto-N-biose/lacto-N-biose I pathway of bifidobacteria. Starting the reaction with 600 mM GlcNAc, 170 mM GalNAc was produced at equilibrium in the presence of catalytic amounts of ATP and UDP-Glc under optimized conditions. GalNAc was separated from GlcNAc using water-eluting cation-exchange chromatography with a commonly available cation-exchange resin. (C) 2011 Elsevier Ltd. All rights reserved.
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页码：2432 / 2436
页数：5
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