Virus Isolation from the First Patient with SARS-CoV-2 in Korea

被引：127
|
作者：
Park, Wan Beom [1 ,2 ]
Kwon, Nak-Jung [3 ]
Choi, Su-Jin [2 ]
Kang, Chang Kyung [1 ]
Choe, Pyoeng Gyun [1 ]
Kim, Jin Yong [4 ]
Yun, Jiyoung [3 ]
Lee, Gir-Won [3 ]
Seong, Moon-Woo [5 ]
Kim, Nam Joong [1 ,2 ]
Seo, Jeong-Sun [3 ,6 ]
Oh, Myoung-don [1 ,2 ]
机构：
[1] Seoul Natl Univ, Dept Internal Med, Coll Med, 101 Daehak Ro, Seoul 03080, South Korea
[2] Seoul Natl Univ Hosp, Lab Infect & Immun, Biomed Res Inst, Seoul, South Korea
[3] Macrogen Inc, Seoul, South Korea
[4] Incheon Med Ctr, Dept Internal Med, Incheon, South Korea
[5] Seoul Natl Univ, Dept Lab Med, Coll Med, Seoul, South Korea
[6] Seoul Natl Univ, Precis Med Ctr, Bundang Hosp, Seongnam, South Korea
来源：
JOURNAL OF KOREAN MEDICAL SCIENCE | 2020年 / 35卷 / 07期
关键词：
2019 Novel Coronavirus; Culture; Microscopy; Electron; Phylogeny; Korea; CORONAVIRUS; ALIGNMENT;
D O I：
10.3346/jkms.2020.35.e84
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Novel coronavirus (SARS-CoV-2) is found to cause a large outbreak started from Wuhan since December 2019 in China and SARS-CoV-2 infections have been reported with epidemiological linkage to China in 25 countries until now. We isolated SARS-CoV-2 from the oropharyngeal sample obtained from the patient with the first laboratory-confirmed SARSCoV-2 infection in Korea. Cytopathic effects of SARS-CoV-2 in the Vero cell cultures were confluent 3 days after the first blind passage of the sample. Coronavirus was confirmed with spherical particle having a fringe reminiscent of crown on transmission electron microscopy. Phylogenetic analyses of whole genome sequences showed that it clustered with other SARS-CoV-2 reported from Wuhan.
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页数：5
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