Structural analysis and CCK-releasing activity of a sulphated polysaccharide from abalone (Haliotis Discus Hannai Ino) viscera

被引：32
|
作者：
Zhu, Bei-wei [1 ]
Li, Dong-mei [1 ]
Zhou, Da-yong [1 ]
Han, Song [2 ]
Yang, Jing-feng [1 ]
Li, Tao [1 ]
Ye, Wen-xiu [1 ]
Greeley, George H. [2 ]
机构：
[1] Dalian Polytech Univ, Sch Food Sci & Technol, Dalian 116034, Peoples R China
[2] Univ Texas Med Branch, Dept Surg, Galveston, TX 77555 USA
来源：
FOOD CHEMISTRY | 2011年 / 125卷 / 04期
基金：
国家高技术研究发展计划(863计划);
关键词：
Abalone (Haliotis Discus Hannai Ino); Viscera; Polysaccharide; Cholecystokinin; PLASMA CHOLECYSTOKININ; FATTY-ACID; PURIFICATION; SUGARS; CELLS; STC-1;
D O I：
10.1016/j.foodchem.2010.10.065
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A fraction of water-soluble sulphated polysaccharide conjugate termed AHP-2 was obtained from abalone (Haliotis Discus Hannai Ino) viscera by protease-assisted aqueous extraction followed by precipitation with ethanol and purification with gel filtration chromatography Analysis by high performance liquid chromatography (HPLC) coupled to a TSK-gel G4000PW(XL) column and gel filtration chromatography on Sepharose CL 6B indicated AHP-2 is homogenous with an average molecular weight (MW) of about 11 0 kDa The structure of AHP-2 was revealed by chemical methods Fourier transform Infrared (FM) spectroscopy and nuclear magnetic resonance (NMR) spectroscopy Results indicated AHP 2 is a heteroglycan consisting of glucose fucose xylose rhamnose and galactose with molar ratio of 1 0 2 0 3 9 6 7 7 4 The backbone of AHP-2 consists of 1 3-linked rhamnose and 1 3 6-linked galactose with glucose fucose xylose and galactose of different linkage types distributing in branched chains Meanwhile AHP-2 was found to increase cholecystokinin (CCK) release in CCK-secreting STC-1 cells (C) 2010 Elsevier Ltd All rights reserved
引用
收藏
页码：1273 / 1278
页数：6
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