Phosphorylation of the Hepatitis B Virus Large Envelope Protein

被引:1
|
作者
Fogeron, Marie-Laure [1 ]
Lecoq, Lauriane [1 ]
Cole, Laura [1 ]
Montserret, Roland [1 ]
David, Guillaume [1 ]
Page, Adeline [2 ]
Delolme, Frederic [2 ]
Nassal, Michael [3 ]
Boeckmann, Anja [1 ]
机构
[1] Univ Lyon, Mol Microbiol & Struct Biochem, Labex Ecofect, CNRS,UMR 5086, Lyon, France
[2] CNRS, SFR BioSci UAR 3444, Prot Sci Facil, INSERM,US8,UCBL,ENS Lyon, Lyon, France
[3] Univ Freiburg, Univ Hosp Freiburg, Dept Med Mol Biol 2, Med Ctr, Freiburg, Germany
关键词
hepatitis B; L HBsAg; phosphorylation; NMR; cell-free (CF) protein synthesis; mass spectrometry; SYNTHESIS SYSTEM; DOMAIN; IDENTIFICATION; PURIFICATION; INFECTION; PRE-S1; MODEL; CORE;
D O I
10.3389/fmolb.2021.821755
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We here establish the phosphorylation sites in the human hepatitis B virus (HBV) large envelope protein (L). L is involved in several functionally important interactions in the viral life cycle, including with the HBV cellular receptor, HBV capsid, Hsc70 chaperone, and cellular membranes during fusion. We have recently shown that cell-free synthesis of the homologous L protein of duck HBV in wheat germ extract results in very similar phosphorylation events to those previously observed in animal cells. Here, we used mass spectrometry and NMR to establish the phosphorylation patterns of human HBV L protein produced by both in vitro cell-free synthesis and in E. coli with the co-expression of the human MAPK14 kinase. While in the avian virus the phosphorylation of L has been shown to be dispensable for infectivity, the identified locations in the human virus protein, both in the PreS1 and PreS2 domains, raise the intriguing possibility that they might play a functional role, since they are found at strategic sites predicted to be involved in L interactions. This would warrant the further investigation of a possible function in virion formation or cell entry.
引用
收藏
页数:10
相关论文
共 50 条
  • [41] HEPATITIS-B ENVELOPE PROTEIN MUTANTS
    MCNAIR, ANB
    VIAGGI, P
    SMITH, HM
    TIBBS, CJ
    NAOUMOV, NV
    WILLIAMS, R
    LANCET, 1995, 346 (8970): : 318 - 319
  • [42] MUTATIONAL ANALYSIS OF THE CYSTEINE RESIDUES IN THE HEPATITIS-B VIRUS SMALL ENVELOPE PROTEIN
    MANGOLD, CMT
    STREECK, RE
    JOURNAL OF VIROLOGY, 1993, 67 (08) : 4588 - 4597
  • [43] Dual topology of the large envelope protein of duck hepatitis B virus: Determinants preventing pre-S translocation and glycosylation
    Swameye, I
    Schaller, H
    JOURNAL OF VIROLOGY, 1997, 71 (12) : 9434 - 9441
  • [45] MAPPING A REGION OF THE LARGE ENVELOPE PROTEIN REQUIRED FOR HEPATITIS-B VIRION MATURATION
    BRUSS, V
    THOMSSEN, R
    JOURNAL OF VIROLOGY, 1994, 68 (03) : 1643 - 1650
  • [46] HEPATITIS DELTA VIRUS - PROTEIN-COMPOSITION OF DELTA ANTIGEN AND ITS HEPATITIS-B VIRUS-DERIVED ENVELOPE
    BONINO, F
    HEERMANN, KH
    RIZZETTO, M
    GERLICH, WH
    JOURNAL OF VIROLOGY, 1986, 58 (03) : 945 - 950
  • [47] HEPATITIS DELTA VIRUS - PROTEIN-COMPOSITION OF DELTA-ANTIGEN AND ITS HEPATITIS-B VIRUS DERIVED ENVELOPE
    BONINO, F
    HEERMANN, KH
    RIZZETTO, M
    GERLICH, WH
    ITALIAN JOURNAL OF GASTROENTEROLOGY, 1986, 18 (05): : 297 - 297
  • [48] MOUSE HEPATITIS-VIRUS GENE 5B PROTEIN IS A NEW VIRION ENVELOPE PROTEIN
    YU, X
    BI, WZ
    WEISS, SR
    LEIBOWITZ, JL
    VIROLOGY, 1994, 202 (02) : 1018 - 1023
  • [49] Quantitative analysis of the interaction between the envelope protein domains and the core protein of human hepatitis B virus
    Choi, KJ
    Lim, CW
    Yoon, MY
    Ahn, BY
    Yu, YG
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2004, 319 (03) : 959 - 966
  • [50] Protein composition of the hepatitis A virus quasi-envelope
    McKnight, Kevin L.
    Xie, Ling
    Gonzalez-Lopez, Olga
    Rivera-Serrano, Efrain E.
    Chen, Xian
    Lemon, Stanley M.
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2017, 114 (25) : 6587 - 6592