Short communication: Effect of inhibition of fatty acid synthase on triglyceride accumulation and effect on lipid metabolism genes in goat mammary epithelial cells

被引：29
|
作者：
Zhu, J. J. [1 ]
Luo, J. [1 ]
Sun, Y. T. [1 ]
Shi, H. B. [1 ]
Li, J. [1 ]
Wu, M. [1 ]
Yu, K. [1 ]
Haile, A. B. [1 ]
Loor, J. J. [2 ,3 ]
机构：
[1] Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi, Peoples R China
[2] Univ Illinois, Dept Anim Sci, Mammalian NutriPhysioGen, Urbana, IL 61801 USA
[3] Univ Illinois, Div Nutr Sci, Urbana, IL 61801 USA
来源：
JOURNAL OF DAIRY SCIENCE | 2015年 / 98卷 / 05期
基金：
中国国家自然科学基金;
关键词：
fatty acid synthase; milk fat; goat mammary epithelial cell; HORMONE-SENSITIVE LIPASE; DIACYLGLYCEROL ACYLTRANSFERASE; MALONYL-COA; GLAND; EXPRESSION; SECRETION; DGAT1; MICE; ADIPOPHILIN; ASSOCIATION;
D O I：
10.3168/jds.2014-8202
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
The role of fatty acid synthase (FASN) on de novo fatty acid synthesis has been well established. In monogastrics, unlike acetyl-coenzyme A carboxylase, FASN is primarily controlled at the transcriptional level. However, no data exist on ruminant mammary cells evaluating effects of FASN knockdown on mRNA expression of lipogenic genes. Inhibition of FASN in mammary cells by C75-mediated interference, a synthetic inhibitor of FASN activity, and short hairpin RNA-mediated interference markedly reduced cellular triglyceride content at least in part by decreasing the expression of genes related to triglyceride synthesis (GPAT, AGPAT6, and DGAT2) and enhancing the expression of lipolysis-related genes (ATGL and HSL). Consistent with the markedly lower expression of genes related to lipid droplet formation and secretion (TIP47, ADFP, BTN1A1, and XDH), cellular lipid droplets also were reduced sharply after incubation with C75 or adenovirus-short-hairpin-RNA. The results underscored the essential role of FASN in the overall process of milk-fat formation in goat mammary epithelial cells.
引用
收藏
页码：3485 / 3491
页数：7
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